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Volume 26
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10.21775/cimb.026.015
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

dCas9: A Versatile Tool for Epigenome Editing

by
Daan J.W. Brocken
1,*,
Mariliis Tark-Dame
2,* and
Remus T. Dame
1,3,*
1
Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands
2
Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
3
Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands
*
Authors to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2018, 26(1), 15-32; https://doi.org/10.21775/cimb.026.015
Submission received: 5 June 2017 / Revised: 9 July 2017 / Accepted: 8 August 2017 / Published: 7 September 2017

Abstract

The epigenome is a heritable layer of information not encoded in the DNA sequence of the genome, but in chemical modifications of DNA or histones. These chemical modifications, together with transcription factors, operate as spatiotemporal regulators of genome activity. Dissecting epigenome function requires controlled site-specific alteration of epigenetic information. Such control can be obtained using designed DNA-binding platforms associated with effector domains to function as targeted transcription factors or epigenetic modifiers. Here, we review the use of dCas9 as a novel and versatile tool for fundamental studies on epigenetic landscapes, chromatin structure and transcription regulation, and the potential of this approach in basic research in these fields.

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MDPI and ACS Style

Brocken, D.J.W.; Tark-Dame, M.; Dame, R.T. dCas9: A Versatile Tool for Epigenome Editing. Curr. Issues Mol. Biol. 2018, 26, 15-32. https://doi.org/10.21775/cimb.026.015

AMA Style

Brocken DJW, Tark-Dame M, Dame RT. dCas9: A Versatile Tool for Epigenome Editing. Current Issues in Molecular Biology. 2018; 26(1):15-32. https://doi.org/10.21775/cimb.026.015

Chicago/Turabian Style

Brocken, Daan J.W., Mariliis Tark-Dame, and Remus T. Dame. 2018. "dCas9: A Versatile Tool for Epigenome Editing" Current Issues in Molecular Biology 26, no. 1: 15-32. https://doi.org/10.21775/cimb.026.015

APA Style

Brocken, D. J. W., Tark-Dame, M., & Dame, R. T. (2018). dCas9: A Versatile Tool for Epigenome Editing. Current Issues in Molecular Biology, 26(1), 15-32. https://doi.org/10.21775/cimb.026.015

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