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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes

by
Henrik Ørum
PNA Diagnostics A/S, Rønnegade 2, 6.floor, 2100 Copenhagen Ø, Denmark
Curr. Issues Mol. Biol. 1999, 1(2), 105-110; https://doi.org/10.21775/cimb.001.105
Submission received: 3 January 1999 / Revised: 10 March 1999 / Accepted: 6 May 1999 / Published: 31 July 1999
Versions Notes

Abstract

The use of affinity tagged PNA capture probes offers an efficient means for the purification of nucleic acids by hybridization. Two different approaches are described. A sequence specific method and a generic method. The sequence specific method requires sequence information on the target and synthesis of a dedicated PNA. It can be used to selectively purify the nucleic acid containing the target from non-related nucleic acids and other cellular components. The generic method uses a "universal" triplex forming PNA and requires no sequence information on the target. It can be used in the bulk purification of large nucleic acids.

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MDPI and ACS Style

Ørum, H. Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes. Curr. Issues Mol. Biol. 1999, 1, 105-110. https://doi.org/10.21775/cimb.001.105

AMA Style

Ørum H. Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes. Current Issues in Molecular Biology. 1999; 1(2):105-110. https://doi.org/10.21775/cimb.001.105

Chicago/Turabian Style

Ørum, Henrik. 1999. "Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes" Current Issues in Molecular Biology 1, no. 2: 105-110. https://doi.org/10.21775/cimb.001.105

APA Style

Ørum, H. (1999). Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes. Current Issues in Molecular Biology, 1(2), 105-110. https://doi.org/10.21775/cimb.001.105

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