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Characterization of N-Linked Glycosylation in a Monoclonal Antibody Produced in NS0 Cells Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Vaccine Analytical Development, Merck Research Laboratories, West Point, PA 19486, USA
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Present Address: Sino Biological, Inc., 14 Zhong He St., BDA, Beijing 100176, China.
Pharmaceuticals 2013, 6(3), 393-406; https://doi.org/10.3390/ph6030393
Received: 29 January 2013 / Accepted: 13 March 2013 / Published: 21 March 2013
(This article belongs to the Special Issue Biologics)
The N-linked glycosylation in recombinant monoclonal antibodies (mAb) occurs at Asn297 on the Fc region in the CH2 domain. Glycosylation heterogeneities have been well documented to affect biological activities such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) through their interaction with Fc-receptors. Hence, it is critical to monitor and characterize the N-linked glycosylation profile in a therapeutic protein such as a mAb for product consistency. In one approach, the glycans are first released from the mAb using an enzyme specific digestion, such as Protein N-Glycosidase F (PNGase) and subsequently they are labeled using a fluorophore, for example, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) . Here we have applied this approach and used Capillary Electrophoresis with Laser-Induced Fluorescence detection (CE-LIF) to analyze a recombinant mAb produced in murine myeloma (NS0) cells. The technique provides short analysis times, efficient separations, and high sensitivity. CE-LIF peak identification was done by a combination of glycan standards and treatment with various exoglycosidases. Furthermore, the APTS-labeled glycans were also analyzed using hydrophilic interaction chromatography (HILIC) high performance liquid chromatography (HPLC) to aid identification of minor peaks by sample collection and off-line mass spectrometry (MS) analysis. View Full-Text
Keywords: CE-LIF; APTS; HILIC-HPLC; glycans; monoclonal antibody NS0; capillary electrophoresis CE-LIF; APTS; HILIC-HPLC; glycans; monoclonal antibody NS0; capillary electrophoresis
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Hamm, M.; Wang, Y.; Rustandi, R.R. Characterization of N-Linked Glycosylation in a Monoclonal Antibody Produced in NS0 Cells Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection. Pharmaceuticals 2013, 6, 393-406.

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