Next Article in Journal
Fabrication of Biochips with Micro Fluidic Channels by Micro End-milling and Powder Blasting
Next Article in Special Issue
Assembling Amperometric Biosensors for Clinical Diagnostics
Previous Article in Journal
Road Asphalt Pavements Analyzed by Airborne Thermal Remote Sensing: Preliminary Results of the Venice Highway
Previous Article in Special Issue
Dependence of Impedance of Embedded Single Cells on Cellular Behaviour
Article Menu

Export Article

Open AccessArticle
Sensors 2008, 8(2), 1297-1307;

Construction of a nrdA::luxCDABE Fusion and Its Use in Escherichia coli as a DNA Damage Biosensor

College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul, Republic of Korea
Diagnostics Group, Institut Pasteur Korea, Hawolgok-dong, Seongbuk-gu, Seoul, Republic of Korea
Author to whom correspondence should be addressed.
Received: 31 January 2008 / Accepted: 21 February 2008 / Published: 22 February 2008
Full-Text   |   PDF [388 KB, uploaded 21 June 2014]


The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E. coli strain, BBTNrdA, harboring a plasmid with the nrdA promoter fused to the luxCDABE operon, was successfully constructed. Its response to various chemicals including genotoxic chemicals substantiates it as a DNA damage biosensor. In characterization, three different classes of toxicants were used: DNA damaging chemicals, oxidative stress chemicals, and phenolics. BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). In contrast, there were no responses from the oxidative stress chemicals and phenolics, except from hydrogen peroxide (H2O2) which is known to cause DNA damage indirectly. Therefore, the results of the study demonstrate that BBTNrdA can be used as a DNA damage biosensor. View Full-Text
Keywords: ribonucleoside diphosphate reductase; DNA damage response; ribonucleoside diphosphate reductase; DNA damage response;
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Hwang, E.T.; Ahn, J.-.M.; Kim, B.C.; Gu, M.B. Construction of a nrdA::luxCDABE Fusion and Its Use in Escherichia coli as a DNA Damage Biosensor. Sensors 2008, 8, 1297-1307.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top