Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Apparatus and Measurements
2.3. Preparation of Modified Electrode
2.4. Sample Preparation
3. Results and Discussion
3.1. Characteristics of Modified Materials
3.2. Electrochemical Studies
3.3. Optimization
3.4. Electrocatalytic Behaviors
3.5. Anti-Interference Ability, and Stability of Biosensor
3.6. Determination of Glu in Plasma
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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pH | E°′ (V) | Linear Range (mM) | LOD (μM) | (mM) |
---|---|---|---|---|
6 | −0.367 | 0.01–1.6 | 18 | 0.645 |
7 | −0.452 | 0.05–1.1 | 57 | 0.955 |
Methods | Linear Range (mM) | LOD (μM) | (mM) | Sensitivity (μA·cm−2·mM−1) |
---|---|---|---|---|
LSV | 0.01–1.6 | 18 | 0.645 | 18 |
DPV | 0.01–3.5 | 17 | 0.119 | 167 |
Working Electrode | Liner Range (mM) | LOD (μM) | (mM) | Sensitivity (μA·mM−1·cm−2) | Reference |
---|---|---|---|---|---|
MPC-CHI-GOD/SPCE | 0.25–3 | 4.1 | 2.1 | 56.12 | [52] |
NF/GOD/IL/mPEG-fMWCNTs/GCE | 0.02–0.95 | 0.2 | 0.143 | - | [34] |
CHI/GOD-HFs/GCE | 0.05–0.5 | 5 | 0.694 | - | [27] |
GOD/PEDOT/CF | 0.5–15 | 6.5 | 8.5 | [48] | |
GOD/CoS-MWCNTs/NF/GCE | 0.008–1.5 | 5 | - | 14.96 | [41] |
PEDOT:PSS/Ti3C2/GQD-GOD/SPCE | 0–0.5 | 65 | - | 21.64 | [49] |
GOD/Au@C/TiO2/FTO | 0.1–1.6 | 42 | - | 29.76 | [50] |
FTO-CNTS/PEI-GOD | 0.07–0.7 | 70 | - | 63.38 | [51] |
GOD/PEDOT:PSS/CNTF | 0.05–0.5 | 43.52 | 1.63 | 43.52 | [42] |
GOD/PtNPs@NG/NF/GCE | 0.005–1.1 | 2 | 0.66 | 20.31 | [53] |
NF-GLA/MWCNTs-BSA-HFs-GOD/GCE | 0.01–3.5 | 17 | 0.119 | 167 | This work |
Sample Number | Glu Found by Commercial Glucose Meter (μM) | Glu Found by Modified Biosensor (μM) | Recovery (%) | RSD |
---|---|---|---|---|
1 | 30 | 29.6 | 98.7 | 1.25 |
2 | 150 | 155.7 | 103.9 | 2.30 |
3 | 500 | 479.2 | 95.9 | 2.80 |
4 | 750 | 750.3 | 100.0 | 2.96 |
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Li, Y.-Y.; Ma, X.-X.; Song, X.-Y.; Ma, L.-L.; Li, Y.-Y.; Meng, X.; Chen, Y.-J.; Xu, K.-X.; Moosavi-Movahedi, A.A.; Xiao, B.-L.; et al. Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors 2023, 23, 3209. https://doi.org/10.3390/s23063209
Li Y-Y, Ma X-X, Song X-Y, Ma L-L, Li Y-Y, Meng X, Chen Y-J, Xu K-X, Moosavi-Movahedi AA, Xiao B-L, et al. Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors. 2023; 23(6):3209. https://doi.org/10.3390/s23063209
Chicago/Turabian StyleLi, Yang-Yang, Xin-Xin Ma, Xin-Yan Song, Lin-Lin Ma, Yu-Ying Li, Xin Meng, Yu-Jie Chen, Ke-Xin Xu, Ali Akbar Moosavi-Movahedi, Bao-Lin Xiao, and et al. 2023. "Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode" Sensors 23, no. 6: 3209. https://doi.org/10.3390/s23063209
APA StyleLi, Y. -Y., Ma, X. -X., Song, X. -Y., Ma, L. -L., Li, Y. -Y., Meng, X., Chen, Y. -J., Xu, K. -X., Moosavi-Movahedi, A. A., Xiao, B. -L., & Hong, J. (2023). Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode. Sensors, 23(6), 3209. https://doi.org/10.3390/s23063209