Next Article in Journal
Siamese Networks-Based People Tracking Using Template Update for 360-Degree Videos Using EAC Format
Next Article in Special Issue
Effect of Low-Doses of Gamma Radiation on Electric Arc-Induced Long Period Fiber Gratings
Previous Article in Journal
A Facile One-Pot Approach to the Synthesis of Gd-Eu Based Metal-Organic Frameworks and Applications to Sensing of Fe3+ and Cr2O72− Ions
Previous Article in Special Issue
Monitoring the Growth of a Microbubble Generated Photothermally onto an Optical Fiber by Means Fabry–Perot Interferometry
Open AccessArticle

Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers

1
Department of Engineering, University of Campania Luigi Vanvitelli, Via Roma 29, 81031 Aversa, Italy
2
Moresense Srl, Filarete Foundation, Viale Ortles 22/4, 20139 Milano, Italy
3
Operative Unit of Chemical-Clinical and Microbiological Analysis, San Luca Hospital, Usl Toscana Nord Ovest, 55100 Lucca, Italy
4
UOC Chirurgia Generale, Ospedale Valtiberina, Usl Toscana Sud-Est, 52037 Sansepolcro, Italy
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Academic Editor: Paulo Caldas
Sensors 2021, 21(5), 1681; https://doi.org/10.3390/s21051681
Received: 3 February 2021 / Revised: 22 February 2021 / Accepted: 24 February 2021 / Published: 1 March 2021
(This article belongs to the Special Issue Optical Fiber Sensors)
The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transcription Polymerase Chain Reaction (RT-PCR) real time analysis that requires specialized equipment, reagents and facilities and typically 3–4 h to perform. Thus, the realization of simple, low-cost, small-size, rapid and point-of-care diagnostics tests has become a global priority. In response to the current need for quick, highly sensitive and on-site detection of the SARS-CoV-2 virus in several aqueous solutions, a specific molecularly imprinted polymer (MIP) receptor has been designed, realized, and combined with an optical sensor. More specifically, the proof of concept of a SARS-CoV-2 sensor has been demonstrated by exploiting a plasmonic plastic optical fiber sensor coupled with a novel kind of synthetic MIP nano-layer, especially designed for the specific recognition of Subunit 1 of the SARS-CoV-2 Spike protein. First, we have tested the effectiveness of the developed MIP receptor to bind the Subunit 1 of the SARS-CoV-2 spike protein, then the results of preliminary tests on SARS-CoV-2 virions, performed on samples of nasopharyngeal (NP) swabs in universal transport medium (UTM) and physiological solution (0.9% NaCl), were compared with those obtained with RT-PCR. According to these preliminary results, the sensitivity of the proposed optical-chemical sensor proved to be higher than the RT-PCR one. Furthermore, a relatively fast response time (about 10 min) to the virus was obtained without the use of additional reagents. View Full-Text
Keywords: SARS-CoV-2; optical-chemical sensors; molecularly imprinted polymers (MIPs); surface plasmon resonance (SPR); optical fiber sensors SARS-CoV-2; optical-chemical sensors; molecularly imprinted polymers (MIPs); surface plasmon resonance (SPR); optical fiber sensors
Show Figures

Figure 1

MDPI and ACS Style

Cennamo, N.; D’Agostino, G.; Perri, C.; Arcadio, F.; Chiaretti, G.; Parisio, E.M.; Camarlinghi, G.; Vettori, C.; Di Marzo, F.; Cennamo, R.; Porto, G.; Zeni, L. Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers. Sensors 2021, 21, 1681. https://doi.org/10.3390/s21051681

AMA Style

Cennamo N, D’Agostino G, Perri C, Arcadio F, Chiaretti G, Parisio EM, Camarlinghi G, Vettori C, Di Marzo F, Cennamo R, Porto G, Zeni L. Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers. Sensors. 2021; 21(5):1681. https://doi.org/10.3390/s21051681

Chicago/Turabian Style

Cennamo, Nunzio; D’Agostino, Girolamo; Perri, Chiara; Arcadio, Francesco; Chiaretti, Guido; Parisio, Eva M.; Camarlinghi, Giulio; Vettori, Chiara; Di Marzo, Francesco; Cennamo, Rosario; Porto, Giovanni; Zeni, Luigi. 2021. "Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers" Sensors 21, no. 5: 1681. https://doi.org/10.3390/s21051681

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop