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Open AccessArticle

High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging

Ministry of Education Key Lab of RF Circuits and Systems, Hangzhou Dianzi University, Hangzhou 310018, China
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Sensors 2021, 21(3), 720; https://doi.org/10.3390/s21030720
Received: 20 December 2020 / Revised: 12 January 2021 / Accepted: 19 January 2021 / Published: 21 January 2021
(This article belongs to the Section Nanosensors)
The lensless on-chip microscope is an emerging technology in the recent decade that can realize the imaging and analysis of biological samples with a wide field-of-view without huge optical devices and any lenses. Because of its small size, low cost, and being easy to hold and operate, it can be used as an alternative tool for large microscopes in resource-poor or remote areas, which is of great significance for the diagnosis, treatment, and prevention of diseases. To improve the low-resolution characteristics of the existing lensless shadow imaging systems and to meet the high-resolution needs of point-of-care testing, here, we propose a high-precision on-chip microscope based on in-line holographic technology. We demonstrated the ability of the iterative phase recovery algorithm to recover sample information and evaluated it with image quality evaluation algorithms with or without reference. The results showed that the resolution of the holographic image after iterative phase recovery is 1.41 times that of traditional shadow imaging. Moreover, we used machine learning tools to identify and count the mixed samples of mouse ascites tumor cells and micro-particles that were iterative phase recovered. The results showed that the on-chip cell counter had high-precision counting characteristics as compared with manual counting of the microscope reference image. Therefore, the proposed high-precision lensless microscope on a chip based on in-line holographic imaging provides one promising solution for future point-of-care testing (POCT). View Full-Text
Keywords: high-resolution; on-chip imaging; lensless holographic microscope; phase recovery; POCT high-resolution; on-chip imaging; lensless holographic microscope; phase recovery; POCT
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MDPI and ACS Style

Huang, X.; Li, Y.; Xu, X.; Wang, R.; Yao, J.; Han, W.; Wei, M.; Chen, J.; Xuan, W.; Sun, L. High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging. Sensors 2021, 21, 720. https://doi.org/10.3390/s21030720

AMA Style

Huang X, Li Y, Xu X, Wang R, Yao J, Han W, Wei M, Chen J, Xuan W, Sun L. High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging. Sensors. 2021; 21(3):720. https://doi.org/10.3390/s21030720

Chicago/Turabian Style

Huang, Xiwei; Li, Yangbo; Xu, Xuefeng; Wang, Renjie; Yao, Jiangfan; Han, Wentao; Wei, Maoyu; Chen, Jin; Xuan, Weipeng; Sun, Lingling. 2021. "High-Precision Lensless Microscope on a Chip Based on In-Line Holographic Imaging" Sensors 21, no. 3: 720. https://doi.org/10.3390/s21030720

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