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Open AccessArticle

Development of a Robust Autofluorescence Lifetime Sensing Method for Use in an Endoscopic Application

1
School of Integrated Design Engineering, Keio University, 3-14-1, Hiyoshi, Yokohama 223-8522, Japan
2
Research Fellow of Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Tokyo 102-0083, Japan
3
Department of System Design Engineering, Keio University, 3-14-1, Hiyoshi, Yokohama 223-8522, Japan
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(7), 1847; https://doi.org/10.3390/s20071847 (registering DOI)
Received: 20 February 2020 / Revised: 17 March 2020 / Accepted: 24 March 2020 / Published: 26 March 2020
(This article belongs to the Special Issue Novel Optical Sensors for Biomedical Application)
Endoscopic autofluorescence lifetime imaging is a promising technique for making quantitative and non-invasive diagnoses of abnormal tissue. However, motion artifacts caused by vibration in the direction perpendicular to the tissue surface in a body makes clinical diagnosis difficult. Thus, this paper proposes a robust autofluorescence lifetime sensing technique with a lens tracking system based on a laser beam spot analysis. Our optical setup can be easily mounted on the head of an endoscope. The variation in distance between the optical system and the target surface is tracked by the change in the spot size of the laser beam captured by the camera, and the lens actuator is feedback-controlled to suppress motion artifacts. The experimental results show that, when using a lens tracking system, the standard deviation of fluorescence lifetime is dramatically reduced. Furthermore, the validity of the proposed method is experimentally confirmed by using a bio-mimicking phantom that replicates the shape, optical parameters, and chemical component distribution of the cancerous tissue.
Keywords: artifacts; autofluorescence lifetime; bio-mimicking phantom; endoscopic application; FAD; motion tracking; TCSPC artifacts; autofluorescence lifetime; bio-mimicking phantom; endoscopic application; FAD; motion tracking; TCSPC
MDPI and ACS Style

Ito, S.; Hashimoto, M.; Taguchi, Y. Development of a Robust Autofluorescence Lifetime Sensing Method for Use in an Endoscopic Application. Sensors 2020, 20, 1847.

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