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Open AccessArticle

Enzymatic Platforms for Sensitive Neurotransmitter Detection

1
Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland
2
Faculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(2), 423; https://doi.org/10.3390/s20020423
Received: 13 November 2019 / Revised: 9 December 2019 / Accepted: 17 December 2019 / Published: 11 January 2020
(This article belongs to the Special Issue New Trends in Electrochemical Sensors for Biomedical Applications)
A convenient electrochemical sensing pathway was investigated for neurotransmitter detection based on newly synthesized silole derivatives and laccase/horseradish-peroxidase-modified platinum (Pt)/gold (Au) electrodes. The miniature neurotransmitter’s biosensors were designed and constructed via the immobilization of laccase in an electroactive layer of the Pt electrode coated with poly(2,6-bis(3,4-ethylenedioxythiophene)-4-methyl-4-octyl-dithienosilole) and laccase for serotonin (5-HT) detection, and a Au electrode modified with the electroconducting polymer poly(2,6-bis(selenophen-2-yl)-4-methyl-4-octyl-dithienosilole), along with horseradish peroxidase (HRP), for dopamine (DA) monitoring. These sensing arrangements utilized the catalytic oxidation of neurotransmitters to reactive quinone derivatives (the oxidation process was provided in the enzymes’ presence). Under the optimized conditions, the analytical performance demonstrated a convenient degree of sensitivity: 0.0369 and 0.0256 μA mM−1 cm−2, selectivity in a broad linear range (0.1–200) × 10−6 M) with detection limits of ≈48 and ≈73 nM (for the serotonin and dopamine biosensors, respectively). Moreover, the method was successfully applied for neurotransmitter determination in the presence of interfering compounds (ascorbic acid, L-cysteine, and uric acid). View Full-Text
Keywords: biosensor; dopamine; horseradish peroxidase; laccase; polymer; serotonin biosensor; dopamine; horseradish peroxidase; laccase; polymer; serotonin
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MDPI and ACS Style

Baluta, S.; Zając, D.; Szyszka, A.; Malecha, K.; Cabaj, J. Enzymatic Platforms for Sensitive Neurotransmitter Detection. Sensors 2020, 20, 423.

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