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Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis

1
Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050 Tomsk, Russia
2
Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Charles University, Albertov 6, 12843 Prague 2, Czech Republic
3
Department of Solid State Chemistry, University of Chemistry and Technology, Prague, Technicka 5, 16628 Prague 6, Czech Republic
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(9), 2103; https://doi.org/10.3390/s19092103
Received: 1 April 2019 / Revised: 1 May 2019 / Accepted: 3 May 2019 / Published: 7 May 2019
(This article belongs to the Section Biosensors)
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Abstract

A new simple electrochemical immunosensor approach for the determination of antibodies to tick-borne encephalitis virus (TBEV) in immunological products was developed and tested. The assay is performed by detecting the silver reduction signal in the bioconjugates with antibodies ([email protected]). Here, signal is read by cathodic linear sweep voltammetry (CLSV) through the detection of silver chloride reduction on a gold–carbon composite electrode (GCCE). Covalent immobilization of the antigen on the electrode surface was performed after thiolation and glutarization of the GCCE. Specific attention has been paid to the selection of conditions for stabilizing both the silver nanoparticles and their [email protected]. A simple flocculation test with NaCl was used to select the concentration of antibodies, and the additional stabilizer bovine serum albumin (BSA) was used for [email protected] preparation. The antibodies to TBEV were quantified in the range from 50 IU·mL−1 to 1600 IU·mL−1, with a detection limit of 50 IU·mL−1. The coefficient of determination (r2) is 0.989. The electrochemical immunosensor was successfully applied to check the quality of immunological products containing IgG antibodies to TBEV. The present work paves the path for a novel method for monitoring TBEV in biological fluids. View Full-Text
Keywords: Bioconjugates; silver nanoparticles; antibodies; tick-borne encephalitis virus; cathodic linear sweep voltammetry (CLSV); gold–carbon composite electrode (GCCE); electrochemical immunosensor Bioconjugates; silver nanoparticles; antibodies; tick-borne encephalitis virus; cathodic linear sweep voltammetry (CLSV); gold–carbon composite electrode (GCCE); electrochemical immunosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Khristunova, Y.; Korotkova, E.; Kratochvil, B.; Barek, J.; Dorozhko, E.; Vyskocil, V.; Plotnikov, E.; Voronova, O.; Sidelnikov, V. Preparation and Investigation of Silver Nanoparticle–Antibody Bioconjugates for Electrochemical Immunoassay of Tick-Borne Encephalitis. Sensors 2019, 19, 2103.

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