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Surface Plasmon Enhanced Light Scattering Biosensing: Size Dependence on the Gold Nanoparticle Tag

1
Future Industries Institute and ARC Centre of Excellence in Convergent Bio and Nano Science and Technology, University of South Australia, Mawson Lakes Campus, Mawson Lakes 5095, Australia
2
Electronics and Photonics Department, Institute of High Performance Computing, Agency for Science, Technology, and Research (A*STAR), Singapore 138632, Singapore
3
SUTD-MIT International Design Center & Science and Math Cluster, Singapore University of Technology and Design, Singapore 487372, Singapore
4
School of Biomedical Engineering, University of Technology Sydney, Sydney 2007, Australia
5
Institute for Biomedical Materials and Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
6
Institute of Comparative Medicine, Yangzhou University, Yangzhou 225009, China
*
Author to whom correspondence should be addressed.
Sensors 2019, 19(2), 323; https://doi.org/10.3390/s19020323
Received: 21 December 2018 / Revised: 11 January 2019 / Accepted: 11 January 2019 / Published: 15 January 2019
(This article belongs to the Section Biosensors)
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PDF [3329 KB, uploaded 15 January 2019]
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Abstract

Surface plasmon enhanced light scattering (SP-LS) is a powerful new sensing SPR modality that yields excellent sensitivity in sandwich immunoassay using spherical gold nanoparticle (AuNP) tags. Towards further improving the performance of SP-LS, we systematically investigated the AuNP size effect. Simulation results indicated an AuNP size-dependent scattered power, and predicted the optimized AuNPs sizes (i.e., 100 and 130 nm) that afford extremely high signal enhancement in SP-LS. The maximum scattered power from a 130 nm AuNP is about 1700-fold higher than that obtained from a 17 nm AuNP. Experimentally, a bio-conjugation protocol was developed by coating the AuNPs with mixture of low and high molecular weight PEG molecules. Optimal IgG antibody bioconjugation conditions were identified using physicochemical characterization and a model dot-blot assay. Aggregation prevented the use of the larger AuNPs in SP-LS experiments. As predicted by simulation, AuNPs with diameters of 50 and 64 nm yielded significantly higher SP-LS signal enhancement in comparison to the smaller particles. Finally, we demonstrated the feasibility of a two-step SP-LS protocol based on a gold enhancement step, aimed at enlarging 36 nm AuNPs tags. This study provides a blue-print for the further development of SP-LS biosensing and its translation in the bioanalytical field. View Full-Text
Keywords: surface plasmon resonance; surface plasmon enhanced light scattering; gold nanoparticles; signal amplification; gold enhancement surface plasmon resonance; surface plasmon enhanced light scattering; gold nanoparticles; signal amplification; gold enhancement
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Yang, C.-T.; Xu, Y.; Pourhassan-Moghaddam, M.; Tran, D.P.; Wu, L.; Zhou, X.; Thierry, B. Surface Plasmon Enhanced Light Scattering Biosensing: Size Dependence on the Gold Nanoparticle Tag. Sensors 2019, 19, 323.

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