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Open AccessArticle

Detection of Metallothionein in Javanese Medaka (Oryzias javanicus), Using a scFv-Immobilized Protein Chip

1
Department of Biomedical Engineering, Dongguk University, Seoul 04620, Korea
2
Faculty of Marine Environmental Science, University of Science and Technology (UST), Geoje 53201, Korea
3
South Sea Environment Research Center, Korea Institute of Ocean Science and Technology (KIOST), Geoje 53201, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Sensors 2018, 18(4), 1069; https://doi.org/10.3390/s18041069
Received: 18 January 2018 / Revised: 16 March 2018 / Accepted: 27 March 2018 / Published: 2 April 2018
(This article belongs to the Special Issue Environmental Monitoring Biosensors)
Environmental pollution by various industrial chemicals and biological agents poses serious risks to human health. Especially, marine contamination by potentially toxic elements (PTEs) has become a global concern in recent years. Many efforts have been undertaken to monitor the PTE contamination of the aquatic environment. However, there are few approaches available to assess the PTE exposure of aquatic organisms. In this research, we developed a strategy to evaluate the heavy metal exposure of marine organisms, by measuring the expression levels of metallothionein protein derived from Oryzias javanicus (OjaMT). OjaMT is a biomarker of heavy metal exposure because the expression level increases upon heavy metal exposure. The developed assay is based on a real-time, label-free surface plasmon resonance (SPR) measurement. Anti-OjaMT antibody and anti-OjaMT single-chain fragment of variable region (scFv) were used as detection probes. Two types of SPR sensor chips were fabricated, by immobilizing antibody or Cys3-tagged scFv (scFv-Cys3) in a controlled orientation and were tested for in situ label-free OjaMT detection. Compared to the antibody-presenting sensor chips, the scFv-presenting sensor chips showed improved performance, displaying enhanced sensitivity and enabling semi-quantitative detection. The portable SPR system combined with scFv-immobilized sensor chips is expected to provide an excellent point-of-care testing system that can monitor target biomarkers in real time. View Full-Text
Keywords: metallothionein; single-chain fragment of variable region (scFv); surface plasmon resonance (SPR); biomarker; sensor chip metallothionein; single-chain fragment of variable region (scFv); surface plasmon resonance (SPR); biomarker; sensor chip
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Lee, E.; Jeon, H.; Kang, C.; Woo, S.; Yum, S.; Kwon, Y. Detection of Metallothionein in Javanese Medaka (Oryzias javanicus), Using a scFv-Immobilized Protein Chip. Sensors 2018, 18, 1069.

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