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Open AccessCommunication

Significant Sensitivity Improvement for Camera-Based Lateral Flow Immunoassay Readers

1
School of Electronic Engineering, Institute of Engineering, Suranaree University of Technology, 111 University Ave., Muang, Nakhon Ratchasima 30000, Thailand
2
Photonics Technology Laboratory, National Electronics and Computer Technology Center, 112 Thailand Science Park, Phahon Yothin Rd., Pathumthai 12120, Thailand
3
National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahon Yothin Rd., Pathumthai 12120, Thailand
*
Author to whom correspondence should be addressed.
Sensors 2018, 18(11), 4026; https://doi.org/10.3390/s18114026
Received: 23 September 2018 / Revised: 16 November 2018 / Accepted: 16 November 2018 / Published: 19 November 2018
(This article belongs to the Special Issue Smartphone-Based Biosensing)
Recent developments in smartphone-based strip readers have further improved the performances of lateral flow test kits. Most smartphone cameras encode an unaltered and nonlinear power-law transfer function that maps the light intensity to a pixel value; this poses some limitations for camera-based strip readers. For faint-color test lines which are almost as white such as with nitrocellulose pads, the slope of the transfer function is low. Therefore, it is difficult to differentiate between the faint test lines and the white background. We show that by manually setting the camera exposure time—instead of using the automatic settings—to the high-slope region of the transfer function, the reader’s sensitivity can be improved. We found that the sensitivity and the limit of detection of the Acidovorax avenae subsp. citrulli (Aac) test kit were enhanced up to 3-fold and 5-fold, respectively, when using the readers at the optimal camera settings, compared to the automatic mode settings. This simple technique can be readily applied to any existing camera-based colorimetric strip reader to significantly improve its performance. View Full-Text
Keywords: lateral flow immunoassays; LFA reader; strip reader; smartphone reader; test kits lateral flow immunoassays; LFA reader; strip reader; smartphone reader; test kits
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MDPI and ACS Style

Saisin, L.; Amarit, R.; Somboonkaew, A.; Gajanandana, O.; Himananto, O.; Sutapun, B. Significant Sensitivity Improvement for Camera-Based Lateral Flow Immunoassay Readers. Sensors 2018, 18, 4026. https://doi.org/10.3390/s18114026

AMA Style

Saisin L, Amarit R, Somboonkaew A, Gajanandana O, Himananto O, Sutapun B. Significant Sensitivity Improvement for Camera-Based Lateral Flow Immunoassay Readers. Sensors. 2018; 18(11):4026. https://doi.org/10.3390/s18114026

Chicago/Turabian Style

Saisin, Lalita; Amarit, Ratthasart; Somboonkaew, Armote; Gajanandana, Oraprapai; Himananto, Orawan; Sutapun, Boonsong. 2018. "Significant Sensitivity Improvement for Camera-Based Lateral Flow Immunoassay Readers" Sensors 18, no. 11: 4026. https://doi.org/10.3390/s18114026

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