Open AccessArticle
Fast Selective Detection of Pyocyanin Using Cyclic Voltammetry
by
Fatima AlZahra’a Alatraktchi 1,2,3,*, Sandra Breum Andersen 2, Helle Krogh Johansen 2,4, Søren Molin 2,3 and Winnie E. Svendsen 1
1
Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800 Kgs. Lyngby, Denmark
2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Allé 6, 2970 Hørsholm, Denmark
3
Department of Systems Biology, Technical University of Denmark, Kemitorvet, 2800 Kgs. Lyngby, Denmark
4
Department of Clinical Microbiology, Afsnit 9301, Rigshospitalet, Juliane Maries Vej 22, 2100 København, Denmark
Cited by 72 | Viewed by 10846
Abstract
Pyocyanin is a virulence factor uniquely produced by the pathogen
Pseudomonas aeruginosa. The fast and selective detection of pyocyanin in clinical samples can reveal important information about the presence of this microorganism in patients. Electrochemical sensing of the redox-active pyocyanin is a
[...] Read more.
Pyocyanin is a virulence factor uniquely produced by the pathogen
Pseudomonas aeruginosa. The fast and selective detection of pyocyanin in clinical samples can reveal important information about the presence of this microorganism in patients. Electrochemical sensing of the redox-active pyocyanin is a route to directly quantify pyocyanin in real time and
in situ in hospitals and clinics. The selective quantification of pyocyanin is, however, limited by other redox-active compounds existing in human fluids and by other metabolites produced by pathogenic bacteria. Here we present a direct selective method to detect pyocyanin in a complex electroactive environment using commercially available electrodes. It is shown that cyclic voltammetry measurements between −1.0 V to 1.0 V reveal a potential detection window of pyocyanin of 0.58–0.82 V that is unaffected by other redox-active interferents. The linear quantification of pyocyanin has an
R2 value of 0.991 across the clinically relevant concentration range of 2–100 µM. The proposed method was tested on human saliva showing a standard deviation of 2.5% ± 1% (
n = 5) from the known added pyocyanin concentration to the samples. This inexpensive procedure is suggested for clinical use in monitoring the presence and state of
P. aeruginosa infection in patients.
Full article
►▼
Show Figures