Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis
Abstract
1. Introduction
2. Results
2.1. Transcriptome Analysis Reveals High, Medium and Low IFNG Subgroups of AD Correlating with Patients’ Ages
2.2. Ex Vivo Secretome Analysis of Lesional T Cells of AD Patients Confirms High and Low IFN-γ Subgroups
2.3. Low and High IFNG AD Subgroups Show Distinct Molecular Pathways with a Strong Activation of Type 1 Immunity in the High IFNG Group
2.4. Digital Cytometry Reveals Higher Amounts of M1 Macrophages, NK Cells and CD4 Memory T Cells in the High IFNG AD Subgroup
2.5. Gene Set Variation Analysis Shows a Higher Cardiovascular Risk, a More Pronounced Activation of the Immune System and a Higher Enrichment of Type 1-Associated Genes in the High IFNG AD Subgroup
2.6. High and Low IFNG AD Subgroups Share a Strong Common Core Signature with Dupilumab Response Genes
3. Discussion
4. Materials and Methods
4.1. Study Cohort
4.2. RNAseq Library Preparation, Sequencing, Mapping and Quantification
4.3. Cluster Analysis (Elbow Method, K-Mean Clustering) and RNAseq Analysis
4.4. Digital Cytometry/Deconvolution—CIBERSORTx
4.5. Gene Set Variation Analysis
4.6. Inform Algorithms and Comparison with Dupilumab Signature
4.7. Visualization
4.8. Analysis of the Secretome of Lesional T Cells
4.9. Language Editing
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wasserer, S.; Jargosch, M.; Mayer, K.E.; Eigemann, J.; Raunegger, T.; Aydin, G.; Eyerich, S.; Biedermann, T.; Eyerich, K.; Lauffer, F. Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis. Int. J. Mol. Sci. 2024, 25, 6158. https://doi.org/10.3390/ijms25116158
Wasserer S, Jargosch M, Mayer KE, Eigemann J, Raunegger T, Aydin G, Eyerich S, Biedermann T, Eyerich K, Lauffer F. Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis. International Journal of Molecular Sciences. 2024; 25(11):6158. https://doi.org/10.3390/ijms25116158
Chicago/Turabian StyleWasserer, Sophia, Manja Jargosch, Kristine E. Mayer, Jessica Eigemann, Theresa Raunegger, Görkem Aydin, Stefanie Eyerich, Tilo Biedermann, Kilian Eyerich, and Felix Lauffer. 2024. "Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis" International Journal of Molecular Sciences 25, no. 11: 6158. https://doi.org/10.3390/ijms25116158
APA StyleWasserer, S., Jargosch, M., Mayer, K. E., Eigemann, J., Raunegger, T., Aydin, G., Eyerich, S., Biedermann, T., Eyerich, K., & Lauffer, F. (2024). Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis. International Journal of Molecular Sciences, 25(11), 6158. https://doi.org/10.3390/ijms25116158