Next Article in Journal
Title IgA Nephropathy and Oral Bacterial Species Related to Dental Caries and Periodontitis
Next Article in Special Issue
Expression of Doublecortin, Glial Fibrillar Acidic Protein, and Vimentin in the Intact Subpallium and after Traumatic Injury to the Pallium in Juvenile Salmon, Oncorhynchus masou
Previous Article in Journal
Structural and Functional Characterization of Four Novel Fibrinogen Mutations in FGB Causing Congenital Fibrinogen Disorder
Previous Article in Special Issue
Using Serum Amino Acids to Predict Traumatic Brain Injury: A Systematic Approach to Utilize Multiple Biomarkers
 
 
Article

Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury

1
Experimental Traumatology Unit, Department of Neuroscience, Karolinska Institute, 171 77 Stockholm, Sweden
2
Section of Neurosurgery, Department of Neuroscience, Uppsala University, 751 85 Uppsala, Sweden
3
Department of Mechanics and Maritime Sciences, Chalmers University of Technology, 412 96 Gothenburg, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Anna-Leena Sirén
Int. J. Mol. Sci. 2022, 23(2), 722; https://doi.org/10.3390/ijms23020722
Received: 21 December 2021 / Revised: 4 January 2022 / Accepted: 8 January 2022 / Published: 10 January 2022
Hippocampal dysfunction contributes to multiple traumatic brain injury sequala. Female rodents’ outcome is superior to male which has been ascribed the neuroprotective sex hormones 17β-estradiol and progesterone. Cytochrome P450 1B1 (CYP1B1) is an oxidative enzyme influencing the neuroinflammatory response by creating inflammatory mediators and metabolizing neuroprotective 17β-estradiol and progesterone. In this study, we aimed to describe hippocampal CYP1B1 mRNA expression, protein presence of CYP1B1 and its key redox partner Cytochrome P450 reductase (CPR) in both sexes, as well as the effect of penetrating traumatic brain injury (pTBI). A total 64 adult Sprague Dawley rats divided by sex received pTBI or sham-surgery and were assigned survival times of 1-, 3-, 5- or 7 days. CYP1B1 mRNA was quantified using in-situ hybridization and immunohistochemistry performed to verify protein colocalization. CYP1B1 mRNA expression was present in all subregions but greatest in CA2 irrespective of sex, survival time or intervention. At 3-, 5- and 7 days post-injury, expression in CA2 was reduced in male rats subjected to pTBI compared to sham-surgery. Females subjected to pTBI instead exhibited increased expression in all CA subregions 3 days post-injury, the only time point expression in CA2 was greater in females than in males. Immunohistochemical analysis confirmed neuronal CYP1B1 protein in all hippocampal subregions, while CPR was limited to CA1 and CA2. CYP1B1 mRNA is constitutively expressed in both sexes. In response to pTBI, females displayed a more urgent but brief regulatory response than males. This indicates there may be sex-dependent differences in CYP1B1 activity, possibly influencing inflammation and neuroprotection in pTBI. View Full-Text
Keywords: traumatic brain injury; sex differences; cytochrome P450 1B1; hippocampus traumatic brain injury; sex differences; cytochrome P450 1B1; hippocampus
Show Figures

Figure 1

MDPI and ACS Style

Lidin, E.; Sköld, M.K.; Angéria, M.; Davidsson, J.; Risling, M. Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury. Int. J. Mol. Sci. 2022, 23, 722. https://doi.org/10.3390/ijms23020722

AMA Style

Lidin E, Sköld MK, Angéria M, Davidsson J, Risling M. Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury. International Journal of Molecular Sciences. 2022; 23(2):722. https://doi.org/10.3390/ijms23020722

Chicago/Turabian Style

Lidin, Erik, Mattias K. Sköld, Maria Angéria, Johan Davidsson, and Mårten Risling. 2022. "Hippocampal Expression of Cytochrome P450 1B1 in Penetrating Traumatic Brain Injury" International Journal of Molecular Sciences 23, no. 2: 722. https://doi.org/10.3390/ijms23020722

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop