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Article

Bidirectional lncRNA Transfer between Cuscuta Parasites and Their Host Plant

State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China
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Author to whom correspondence should be addressed.
Academic Editor: Pil Joon Seo
Int. J. Mol. Sci. 2022, 23(1), 561; https://doi.org/10.3390/ijms23010561
Received: 15 November 2021 / Revised: 28 December 2021 / Accepted: 2 January 2022 / Published: 5 January 2022
(This article belongs to the Collection Recent Advances in Plant Molecular Science in China 2021)
Dodder species (Cuscuta spp.) are holoparasites that have extensive material exchange with their host plants through vascular connections. Recent studies on cross-species transfer have provided breakthrough insights, but little is known about the interaction mechanisms of the inter-plant mobile substances in parasitic systems. We sequenced the transcriptomes of dodder growing on soybean hosts to characterize the long non-coding RNA (lncRNA) transfer between the two species, and found that lncRNAs can move in high numbers (365 dodder lncRNAs and 14 soybean lncRNAs) in a bidirectional manner. Reverse transcription-polymerase chain reaction further confirmed that individual lncRNAs were trafficked in the dodder–soybean parasitic system. To reveal the potential functions of mobile transcripts, the Gene Ontology terms of mobile lncRNA target genes were predicted, and mobile dodder target genes were found to be mainly enriched in “metabolic process”, “catalytic activity”, “signaling”, and “response to stimulus” categories, whereas mobile soybean target genes were enriched in organelle-related categories, indicating that specific mobile lncRNAs may be important in regulating dodder parasitism. Our findings reveal that lncRNAs are transferred between dodder and its host soybean plants, which may act as critical regulators to coordinate the host–dodder interaction at the whole parasitic level. View Full-Text
Keywords: Cuscuta; host plants; long non-coding RNA transfer; transcriptome sequencing; interaction network Cuscuta; host plants; long non-coding RNA transfer; transcriptome sequencing; interaction network
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MDPI and ACS Style

Wu, Y.; Luo, D.; Fang, L.; Zhou, Q.; Liu, W.; Liu, Z. Bidirectional lncRNA Transfer between Cuscuta Parasites and Their Host Plant. Int. J. Mol. Sci. 2022, 23, 561. https://doi.org/10.3390/ijms23010561

AMA Style

Wu Y, Luo D, Fang L, Zhou Q, Liu W, Liu Z. Bidirectional lncRNA Transfer between Cuscuta Parasites and Their Host Plant. International Journal of Molecular Sciences. 2022; 23(1):561. https://doi.org/10.3390/ijms23010561

Chicago/Turabian Style

Wu, Yuguo, Dong Luo, Longfa Fang, Qiang Zhou, Wenxian Liu, and Zhipeng Liu. 2022. "Bidirectional lncRNA Transfer between Cuscuta Parasites and Their Host Plant" International Journal of Molecular Sciences 23, no. 1: 561. https://doi.org/10.3390/ijms23010561

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