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Article

Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana

1
Department of Crop Science, Federal University of Rio Grande do Sul, Porto Alegre, RS 91540-000, Brazil
2
Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany
3
Institute of Experimental Botany, Czech Academy Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, CZ-77900 Olomouc, Czech Republic
*
Authors to whom correspondence should be addressed.
Academic Editor: Frank M. You
Int. J. Mol. Sci. 2021, 22(7), 3314; https://doi.org/10.3390/ijms22073314
Received: 20 February 2021 / Revised: 17 March 2021 / Accepted: 20 March 2021 / Published: 24 March 2021
Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA-directed DNA Methylation (RdDM) pathways modulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild-type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4-D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in REPRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Transcriptomic analysis of the imazethapyr-treated wild type and ros1 plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid biosynthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1-dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment. View Full-Text
Keywords: epigenetics; herbicide resistance; chromatin mutants; ROS1; imazethapyr; glyphosate; 2,4-D epigenetics; herbicide resistance; chromatin mutants; ROS1; imazethapyr; glyphosate; 2,4-D
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MDPI and ACS Style

Markus, C.; Pecinka, A.; Merotto, A., Jr. Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana. Int. J. Mol. Sci. 2021, 22, 3314. https://doi.org/10.3390/ijms22073314

AMA Style

Markus C, Pecinka A, Merotto A Jr.. Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22(7):3314. https://doi.org/10.3390/ijms22073314

Chicago/Turabian Style

Markus, Catarine, Ales Pecinka, and Aldo Merotto Jr. 2021. "Insights into the Role of Transcriptional Gene Silencing in Response to Herbicide-Treatments in Arabidopsis thaliana" International Journal of Molecular Sciences 22, no. 7: 3314. https://doi.org/10.3390/ijms22073314

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