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Article

The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice

1
Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA
2
Richard L. Roudebush VA Medical Center, Indianapolis, IN 46202, USA
3
Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Maria Letizia Trincavelli, Claudia Martini and Diego La Mendola
Int. J. Mol. Sci. 2021, 22(20), 11097; https://doi.org/10.3390/ijms222011097
Received: 21 September 2021 / Revised: 10 October 2021 / Accepted: 10 October 2021 / Published: 14 October 2021
(This article belongs to the Special Issue Angiogenesis in Disease)
Angiogenesis is critical for successful fracture healing. Age-related alterations in endothelial cells (ECs) may cause impaired bone healing. Therefore, examining therapeutic treatments to improve angiogenesis in aging may enhance bone healing. Sirtuin 1 (SIRT1) is highly expressed in ECs and its activation is known to counteract aging. Here, we examined the effects of SRT1720 treatment (SIRT1 activator) on the growth and function of bone marrow and lung ECs (BMECs and LECs, respectively), derived from young (3–4 month) and old (20–24 month) mice. While aging did not alter EC proliferation, treatment with SRT1720 significantly increased proliferation of all LECs. However, SRT1720 only increased proliferation of old female BMECs. Vessel-like tube assays showed similar vessel-like structures between young and old LECs and BMECs from both male and female mice. SRT1720 significantly improved vessel-like structures in all LECs. No age, sex, or treatment differences were found in migration related parameters of LECs. In males, old BMECs had greater migration rates than young BMECs, whereas in females, old BMECs had lower migration rates than young BMECs. Collectively, our data suggest that treatment with SRT1720 appears to enhance the angiogenic potential of LECs irrespective of age or sex. However, its role in BMECs is sex- and age-dependent. View Full-Text
Keywords: angiogenesis; endothelial cells; bone; lungs; aging; Sirtuin 1 angiogenesis; endothelial cells; bone; lungs; aging; Sirtuin 1
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MDPI and ACS Style

Dadwal, U.C.; Bhatti, F.U.R.; Awosanya, O.D.; Staut, C.d.A.; Nagaraj, R.U.; Perugini, A.J., III; Tewari, N.P.; Valuch, C.R.; Sun, S.; Mendenhall, S.K.; Zhou, D.; Mostardo, S.L.; Blosser, R.J.; Li, J.; Kacena, M.A. The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice. Int. J. Mol. Sci. 2021, 22, 11097. https://doi.org/10.3390/ijms222011097

AMA Style

Dadwal UC, Bhatti FUR, Awosanya OD, Staut CdA, Nagaraj RU, Perugini AJ III, Tewari NP, Valuch CR, Sun S, Mendenhall SK, Zhou D, Mostardo SL, Blosser RJ, Li J, Kacena MA. The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice. International Journal of Molecular Sciences. 2021; 22(20):11097. https://doi.org/10.3390/ijms222011097

Chicago/Turabian Style

Dadwal, Ushashi C., Fazal U.R. Bhatti, Olatundun D. Awosanya, Caio d.A. Staut, Rohit U. Nagaraj, Anthony J. Perugini III, Nikhil P. Tewari, Conner R. Valuch, Seungyup Sun, Stephen K. Mendenhall, Donghui Zhou, Sarah L. Mostardo, Rachel J. Blosser, Jiliang Li, and Melissa A. Kacena 2021. "The Effects of SRT1720 Treatment on Endothelial Cells Derived from the Lung and Bone Marrow of Young and Aged, Male and Female Mice" International Journal of Molecular Sciences 22, no. 20: 11097. https://doi.org/10.3390/ijms222011097

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