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Article

Telomeric-Like Repeats Flanked by Sequences Retrotranscribed from the Telomerase RNA Inserted at DNA Double-Strand Break Sites during Vertebrate Genome Evolution

Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy
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Academic Editors: Miroslav Plohl, Eva Šatović and Raquel Chaves
Int. J. Mol. Sci. 2021, 22(20), 11048; https://doi.org/10.3390/ijms222011048
Received: 9 September 2021 / Revised: 8 October 2021 / Accepted: 11 October 2021 / Published: 13 October 2021
(This article belongs to the Special Issue Repetitive DNA Sequences in Eukaryotic Genomes)
Interstitial telomeric sequences (ITSs) are stretches of telomeric-like repeats located at internal chromosomal sites. We previously demonstrated that ITSs have been inserted during the repair of DNA double-strand breaks in the course of evolution and that some rodent ITSs, called TERC-ITSs, are flanked by fragments retrotranscribed from the telomerase RNA component (TERC). In this work, we carried out an extensive search of TERC-ITSs in 30 vertebrate genomes and identified 41 such loci in 22 species, including in humans and other primates. The fragment retrotranscribed from the TERC RNA varies in different lineages and its sequence seems to be related to the organization of TERC. Through comparative analysis of TERC-ITSs with orthologous empty loci, we demonstrated that, at each locus, the TERC-like sequence and the ITS have been inserted in one step in the course of evolution. Our findings suggest that telomerase participated in a peculiar pathway of DNA double-strand break repair involving retrotranscription of its RNA component and that this mechanism may be active in all vertebrate species. These results add new evidence to the hypothesis that RNA-templated DNA repair mechanisms are active in vertebrate cells. View Full-Text
Keywords: repetitive DNA sequences; telomerase; telomerase RNA; telomeric repeats; interstitial telomeres; genome evolution; vertebrates; DNA repair repetitive DNA sequences; telomerase; telomerase RNA; telomeric repeats; interstitial telomeres; genome evolution; vertebrates; DNA repair
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MDPI and ACS Style

Sola, L.; Nergadze, S.G.; Cappelletti, E.; Piras, F.M.; Giulotto, E.; Santagostino, M. Telomeric-Like Repeats Flanked by Sequences Retrotranscribed from the Telomerase RNA Inserted at DNA Double-Strand Break Sites during Vertebrate Genome Evolution. Int. J. Mol. Sci. 2021, 22, 11048. https://doi.org/10.3390/ijms222011048

AMA Style

Sola L, Nergadze SG, Cappelletti E, Piras FM, Giulotto E, Santagostino M. Telomeric-Like Repeats Flanked by Sequences Retrotranscribed from the Telomerase RNA Inserted at DNA Double-Strand Break Sites during Vertebrate Genome Evolution. International Journal of Molecular Sciences. 2021; 22(20):11048. https://doi.org/10.3390/ijms222011048

Chicago/Turabian Style

Sola, Lorenzo, Solomon G. Nergadze, Eleonora Cappelletti, Francesca M. Piras, Elena Giulotto, and Marco Santagostino. 2021. "Telomeric-Like Repeats Flanked by Sequences Retrotranscribed from the Telomerase RNA Inserted at DNA Double-Strand Break Sites during Vertebrate Genome Evolution" International Journal of Molecular Sciences 22, no. 20: 11048. https://doi.org/10.3390/ijms222011048

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