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Article

Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System?

1
Department of Biology and Neuroscience Program, Bowdoin College, Brunswick, ME 04011, USA
2
USDA-ARS, Maricopa, AZ 85138, USA
3
Pacific Biosciences Research Center, University of Hawaii at Manoa, Honolulu, HI 96822, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Erik Johnson
Int. J. Mol. Sci. 2021, 22(16), 8703; https://doi.org/10.3390/ijms22168703
Received: 8 June 2021 / Revised: 26 July 2021 / Accepted: 7 August 2021 / Published: 13 August 2021
(This article belongs to the Special Issue Neuropeptides, Receptors, and Behavior)
Central pattern generators produce rhythmic behaviors independently of sensory input; however, their outputs can be modulated by neuropeptides, thereby allowing for functional flexibility. We investigated the effects of C-type allatostatins (AST-C) on the cardiac ganglion (CG), which is the central pattern generator that controls the heart of the American lobster, Homarus americanus, to identify the biological mechanism underlying the significant variability in individual responses to AST-C. We proposed that the presence of multiple receptors, and thus differential receptor distribution, was at least partly responsible for this observed variability. Using transcriptome mining and PCR-based cloning, we identified four AST-C receptors (ASTCRs) in the CG; we then characterized their cellular localization, binding potential, and functional activation. Only two of the four receptors, ASTCR1 and ASTCR2, were fully functional GPCRs that targeted to the cell surface and were activated by AST-C peptides in our insect cell expression system. All four, however, were amplified from CG cDNAs. Following the confirmation of ASTCR expression, we used physiological and bioinformatic techniques to correlate receptor expression with cardiac responses to AST-C across individuals. Expression of ASTCR1 in the CG showed a negative correlation with increasing contraction amplitude in response to AST-C perfusion through the lobster heart, suggesting that the differential expression of ASTCRs within the CG is partly responsible for the specific physiological response to AST-C exhibited by a given individual lobster. View Full-Text
Keywords: allatostatin C; allatostatin C receptors; neuromodulation; cardiac ganglion; crustacean allatostatin C; allatostatin C receptors; neuromodulation; cardiac ganglion; crustacean
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MDPI and ACS Style

Muscato, A.J.; Walsh, P.; Pong, S.; Pupo, A.; Gross, R.J.; Christie, A.E.; Hull, J.J.; Dickinson, P.S. Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System? Int. J. Mol. Sci. 2021, 22, 8703. https://doi.org/10.3390/ijms22168703

AMA Style

Muscato AJ, Walsh P, Pong S, Pupo A, Gross RJ, Christie AE, Hull JJ, Dickinson PS. Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System? International Journal of Molecular Sciences. 2021; 22(16):8703. https://doi.org/10.3390/ijms22168703

Chicago/Turabian Style

Muscato, Audrey J., Patrick Walsh, Sovannarath Pong, Alixander Pupo, Roni J. Gross, Andrew E. Christie, J. J. Hull, and Patsy S. Dickinson 2021. "Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System?" International Journal of Molecular Sciences 22, no. 16: 8703. https://doi.org/10.3390/ijms22168703

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