Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments

Ito, Yoko; Esnay, Nicolas; Fougère, Louise; Platre, Matthieu Pierre; Cordelières, Fabrice; Jaillais, Yvon; Boutté, Yohann

doi:10.3390/ijms22168450

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Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments

Yoko Ito

^1,†,

Nicolas Esnay

^1,2,†

Louise Fougère

¹,

Matthieu Pierre Platre

^3,4,

Fabrice Cordelières

⁵

Yvon Jaillais

and

Yohann Boutté

^1,*

Laboratoire de Biogenèse Membranaire, Université de Bordeaux, CNRS, 33140 Villenave d’Ornon, France

BioDiscovery Institute, Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA

Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon1, CNRS, INRAE, 69342 Lyon, France

⁴

Plant Molecular and Cellular Biology Laboratory and Integrative Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA

⁵

Bordeaux Imaging Center, Université de Bordeaux, CNRS, 33000 Bordeaux, France

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Academic Editor: Paola Giussani

Int. J. Mol. Sci. 2021, 22(16), 8450; https://doi.org/10.3390/ijms22168450

Received: 25 June 2021 / Revised: 28 July 2021 / Accepted: 3 August 2021 / Published: 6 August 2021

(This article belongs to the Special Issue Sphingolipid Metabolism and Signaling in Diseases)

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Abstract

A main characteristic of sphingolipids is the presence of a very long chain fatty acid (VLCFA) whose function in cellular processes is not yet fully understood. VLCFAs of sphingolipids are involved in the intracellular traffic to the vacuole and the maturation of early endosomes into late endosomes is one of the major pathways for vacuolar traffic. Additionally, the anionic phospholipid phosphatidylinositol-3-phosphate (PtdIns (3)P or PI3P) is involved in protein sorting and recruitment of small GTPase effectors at late endosomes/multivesicular bodies (MVBs) during vacuolar trafficking. In contrast to animal cells, PI3P mainly localizes to late endosomes in plant cells and to a minor extent to a discrete sub-domain of the plant’s early endosome (EE)/trans-Golgi network (TGN) where the endosomal maturation occurs. However, the mechanisms that control the relative levels of PI3P between TGN and MVBs are unknown. Using metazachlor, an inhibitor of VLCFA synthesis, we found that VLCFAs are involved in the TGN/MVB distribution of PI3P. This effect is independent from either synthesis of PI3P by PI3-kinase or degradation of PI(3,5)P₂ into PI3P by the SUPPRESSOR OF ACTIN1 (SAC1) phosphatase. Using high-resolution live cell imaging microscopy, we detected transient associations between TGNs and MVBs but VLCFAs are not involved in those interactions. Nonetheless, our results suggest that PI3P might be transferable from TGN to MVBs and that VLCFAs act in this process. View Full-Text

Keywords: very-long chain fatty acids; phosphatidylinositol-3-phosphate; trans-Golgi network; multivesicular bodies; endosomes; vacuole

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MDPI and ACS Style

Ito, Y.; Esnay, N.; Fougère, L.; Platre, M.P.; Cordelières, F.; Jaillais, Y.; Boutté, Y. Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments. Int. J. Mol. Sci. 2021, 22, 8450. https://doi.org/10.3390/ijms22168450

AMA Style

Ito Y, Esnay N, Fougère L, Platre MP, Cordelières F, Jaillais Y, Boutté Y. Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments. International Journal of Molecular Sciences. 2021; 22(16):8450. https://doi.org/10.3390/ijms22168450

Chicago/Turabian Style

Ito, Yoko, Nicolas Esnay, Louise Fougère, Matthieu Pierre Platre, Fabrice Cordelières, Yvon Jaillais, and Yohann Boutté. 2021. "Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments" International Journal of Molecular Sciences 22, no. 16: 8450. https://doi.org/10.3390/ijms22168450

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Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments

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