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Article

Vitamin C—Protective Role in Oxidative Stress Conditions Induced in Human Normal Colon Cells by Label-Free Raman Spectroscopy and Imaging

Laboratory of Laser Molecular Spectroscopy, Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland
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Academic Editors: Peter J. K. Kuppen and Carmine Stolfi
Int. J. Mol. Sci. 2021, 22(13), 6928; https://doi.org/10.3390/ijms22136928
Received: 22 April 2021 / Revised: 9 June 2021 / Accepted: 22 June 2021 / Published: 28 June 2021
(This article belongs to the Special Issue Biomarkers of Colorectal Cancer)
Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer can detect it at an advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise, and unambiguous differentiation of healthy and cancerous samples. The most important advantage of Raman spectroscopy is the ability to identify biomarkers that help in the differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for lipids, proteins, and DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, and normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS (Reactive Oxygen Species). Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal ROS-injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis. View Full-Text
Keywords: colon cancer; Raman spectroscopy; Raman imaging; biomarkers; biomedical imaging; cell culture; supplementation; vitamin C colon cancer; Raman spectroscopy; Raman imaging; biomarkers; biomedical imaging; cell culture; supplementation; vitamin C
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MDPI and ACS Style

Beton, K.; Brozek-Pluska, B. Vitamin C—Protective Role in Oxidative Stress Conditions Induced in Human Normal Colon Cells by Label-Free Raman Spectroscopy and Imaging. Int. J. Mol. Sci. 2021, 22, 6928. https://doi.org/10.3390/ijms22136928

AMA Style

Beton K, Brozek-Pluska B. Vitamin C—Protective Role in Oxidative Stress Conditions Induced in Human Normal Colon Cells by Label-Free Raman Spectroscopy and Imaging. International Journal of Molecular Sciences. 2021; 22(13):6928. https://doi.org/10.3390/ijms22136928

Chicago/Turabian Style

Beton, Karolina, and Beata Brozek-Pluska. 2021. "Vitamin C—Protective Role in Oxidative Stress Conditions Induced in Human Normal Colon Cells by Label-Free Raman Spectroscopy and Imaging" International Journal of Molecular Sciences 22, no. 13: 6928. https://doi.org/10.3390/ijms22136928

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