Epigallocatechin-3-Gallate Modulates Postoperative Pain by Regulating Biochemical and Molecular Pathways
Abstract
:1. Introduction
2. Results
2.1. Effect of EGCG Administration on Mechanical Allodynia, Thermal Hyperalgesia, and Motor Dysfunction Induced by PO Pain
2.2. Effect of EGCG Administration on WNT/FZ/β-Catenin Pathway Activation Induced by PO Pain
2.3. Effect of EGCG Administration on GFAP and Iba-1 Activation Induced by PO Pain
2.4. Effect of EGCG Administration on pNR2B, pPKCγ, and pCREB Expression Induced by PO Pain
2.5. Effect of EGCG Administration on Cytokines and NFkB Pathway Activation Induced by PO Pain
2.6. Effect of EGCG Administration on iNOS, COX-2 and PGE2 Expression Induced by PO Pain
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Surgical Procedures
4.3. Experimental Groups
4.4. Behavioural Analysis
4.4.1. Mechanical Hyperalgesia
4.4.2. Thermal Hyperalgesia
4.4.3. Motor Coordination
4.5. Immunohistochemical Analysis
4.6. Western Blot Analysis
4.7. ELISA
4.8. Statistical Evaluation
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Siracusa, R.; Monaco, F.; D’Amico, R.; Genovese, T.; Cordaro, M.; Interdonato, L.; Gugliandolo, E.; Peritore, A.F.; Crupi, R.; Cuzzocrea, S.; et al. Epigallocatechin-3-Gallate Modulates Postoperative Pain by Regulating Biochemical and Molecular Pathways. Int. J. Mol. Sci. 2021, 22, 6879. https://doi.org/10.3390/ijms22136879
Siracusa R, Monaco F, D’Amico R, Genovese T, Cordaro M, Interdonato L, Gugliandolo E, Peritore AF, Crupi R, Cuzzocrea S, et al. Epigallocatechin-3-Gallate Modulates Postoperative Pain by Regulating Biochemical and Molecular Pathways. International Journal of Molecular Sciences. 2021; 22(13):6879. https://doi.org/10.3390/ijms22136879
Chicago/Turabian StyleSiracusa, Rosalba, Francesco Monaco, Ramona D’Amico, Tiziana Genovese, Marika Cordaro, Livia Interdonato, Enrico Gugliandolo, Alessio Filippo Peritore, Rosalia Crupi, Salvatore Cuzzocrea, and et al. 2021. "Epigallocatechin-3-Gallate Modulates Postoperative Pain by Regulating Biochemical and Molecular Pathways" International Journal of Molecular Sciences 22, no. 13: 6879. https://doi.org/10.3390/ijms22136879