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Impaired Leptin Signalling in Obesity: Is Leptin a New Thermolipokine?
 
 
Article

Caloric Restriction and Hypothalamic Leptin Gene Therapy Have Differential Effects on Energy Partitioning in Adult Female Rats

1
Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA
2
Biostatistics Program, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR 97331, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Oreste Gualillo
Int. J. Mol. Sci. 2021, 22(13), 6789; https://doi.org/10.3390/ijms22136789
Received: 28 March 2021 / Revised: 4 June 2021 / Accepted: 18 June 2021 / Published: 24 June 2021
(This article belongs to the Special Issue Leptin–Metabolic Programming and Its Endocrine Signals)
Dieting is a common but often ineffective long-term strategy for preventing weight gain. Similar to humans, adult rats exhibit progressive weight gain. The adipokine leptin regulates appetite and energy expenditure but hyperleptinemia is associated with leptin resistance. Here, we compared the effects of increasing leptin levels in the hypothalamus using gene therapy with conventional caloric restriction on weight gain, food consumption, serum leptin and adiponectin levels, white adipose tissue, marrow adipose tissue, and bone in nine-month-old female Sprague-Dawley rats. Rats (n = 16) were implanted with a cannula in the 3rd ventricle of the hypothalamus and injected with a recombinant adeno-associated virus, encoding the rat gene for leptin (rAAV-Lep), and maintained on standard rat chow for 18 weeks. A second group (n = 15) was calorically-restricted to match the weight of the rAAV-Lep group. Both approaches prevented weight gain, and no differences in bone were detected. However, calorically-restricted rats consumed 15% less food and had lower brown adipose tissue Ucp-1 mRNA expression than rAAV-Lep rats. Additionally, calorically-restricted rats had higher abdominal white adipose tissue mass, higher serum leptin and adiponectin levels, and higher marrow adiposity. Caloric restriction and hypothalamic leptin gene therapy, while equally effective in preventing weight gain, differ in their effects on energy intake, energy expenditure, adipokine levels, and body composition. View Full-Text
Keywords: obesity; marrow adipose tissue; white adipose tissue; Ucp-1; leptin; adiponectin; bone architecture obesity; marrow adipose tissue; white adipose tissue; Ucp-1; leptin; adiponectin; bone architecture
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MDPI and ACS Style

Turner, R.T.; Wong, C.P.; Fosse, K.M.; Branscum, A.J.; Iwaniec, U.T. Caloric Restriction and Hypothalamic Leptin Gene Therapy Have Differential Effects on Energy Partitioning in Adult Female Rats. Int. J. Mol. Sci. 2021, 22, 6789. https://doi.org/10.3390/ijms22136789

AMA Style

Turner RT, Wong CP, Fosse KM, Branscum AJ, Iwaniec UT. Caloric Restriction and Hypothalamic Leptin Gene Therapy Have Differential Effects on Energy Partitioning in Adult Female Rats. International Journal of Molecular Sciences. 2021; 22(13):6789. https://doi.org/10.3390/ijms22136789

Chicago/Turabian Style

Turner, Russell T., Carmen P. Wong, Kristina M. Fosse, Adam J. Branscum, and Urszula T. Iwaniec. 2021. "Caloric Restriction and Hypothalamic Leptin Gene Therapy Have Differential Effects on Energy Partitioning in Adult Female Rats" International Journal of Molecular Sciences 22, no. 13: 6789. https://doi.org/10.3390/ijms22136789

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