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Article

Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1

1
Division of Applied Life Science (BK21 Four Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Korea
2
Department of Molecular and Cell Biology, University of Cape Town, Cape Town 7700, South Africa
3
Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
4
Division of Life Science, Gyeongsang National University, Jinju 52828, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Frank M. You
Int. J. Mol. Sci. 2021, 22(12), 6427; https://doi.org/10.3390/ijms22126427
Received: 8 May 2021 / Revised: 13 June 2021 / Accepted: 14 June 2021 / Published: 15 June 2021
Plant immunity is mediated in large part by specific interactions between a host resistance protein and a pathogen effector protein, named effector-triggered immunity (ETI). ETI needs to be tightly controlled both positively and negatively to enable normal plant growth because constitutively activated defense responses are detrimental to the host. In previous work, we reported that mutations in SUPPRESSOR OF rps4-RLD1 (SRFR1), identified in a suppressor screen, reactivated EDS1-dependent ETI to Pseudomonas syringae pv. tomato (Pto) DC3000. Besides, mutations in SRFR1 boosted defense responses to the generalist chewing insect Spodoptera exigua and the sugar beet cyst nematode Heterodera schachtii. Here, we show that mutations in SRFR1 enhance susceptibility to the fungal necrotrophs Fusarium oxysporum f. sp. lycopersici (FOL) and Botrytis cinerea in Arabidopsis. To translate knowledge obtained in AtSRFR1 research to crops, we generated SlSRFR1 alleles in tomato using a CRISPR/Cas9 system. Interestingly, slsrfr1 mutants increased expression of SA-pathway defense genes and enhanced resistance to Pto DC3000. In contrast, slsrfr1 mutants elevated susceptibility to FOL. Together, these data suggest that SRFR1 is functionally conserved in both Arabidopsis and tomato and functions antagonistically as a negative regulator to (hemi-) biotrophic pathogens and a positive regulator to necrotrophic pathogens. View Full-Text
Keywords: SRFR1; CRISPR/Cas9; tomato; fungal necrotrophs; plant resistance SRFR1; CRISPR/Cas9; tomato; fungal necrotrophs; plant resistance
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MDPI and ACS Style

Son, G.H.; Moon, J.; Shelake, R.M.; Vuong, U.T.; Ingle, R.A.; Gassmann, W.; Kim, J.-Y.; Kim, S.H. Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1. Int. J. Mol. Sci. 2021, 22, 6427. https://doi.org/10.3390/ijms22126427

AMA Style

Son GH, Moon J, Shelake RM, Vuong UT, Ingle RA, Gassmann W, Kim J-Y, Kim SH. Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1. International Journal of Molecular Sciences. 2021; 22(12):6427. https://doi.org/10.3390/ijms22126427

Chicago/Turabian Style

Son, Geon Hui, Jiyun Moon, Rahul Mahadev Shelake, Uyen Thi Vuong, Robert A. Ingle, Walter Gassmann, Jae-Yean Kim, and Sang Hee Kim. 2021. "Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1" International Journal of Molecular Sciences 22, no. 12: 6427. https://doi.org/10.3390/ijms22126427

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