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Article

Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls

1
Aix Marseille Univ, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille (CINAM), Turing Centre for Living Systems, 13009 Marseille, France
2
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France
3
Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, France
*
Authors to whom correspondence should be addressed.
Academic Editors: Agnes Csiszár and Bernd Hoffmann
Int. J. Mol. Sci. 2021, 22(12), 6335; https://doi.org/10.3390/ijms22126335
Received: 28 April 2021 / Revised: 26 May 2021 / Accepted: 8 June 2021 / Published: 13 June 2021
(This article belongs to the Special Issue Functionalized Liposomes)
Giant unilamellar vesicles (GUV) are powerful tools to explore physics and biochemistry of the cell membrane in controlled conditions. For example, GUVs were extensively used to probe cell adhesion, but often using non-physiological linkers, due to the difficulty of incorporating transmembrane adhesion proteins into model membranes. Here we describe a new protocol for making GUVs incorporating the transmembrane protein integrin using gel-assisted swelling. We report an optimised protocol, enumerating the pitfalls encountered and precautions to be taken to maintain the robustness of the protocol. We characterise intermediate steps of small proteoliposome formation and the final formed GUVs. We show that the integrin molecules are successfully incorporated and are functional. View Full-Text
Keywords: in vitro reconstitution; liposome; functionalised GUVs; adhesion in vitro reconstitution; liposome; functionalised GUVs; adhesion
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MDPI and ACS Style

Souissi, M.; Pernier, J.; Rossier, O.; Giannone, G.; Le Clainche, C.; Helfer, E.; Sengupta, K. Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls. Int. J. Mol. Sci. 2021, 22, 6335. https://doi.org/10.3390/ijms22126335

AMA Style

Souissi M, Pernier J, Rossier O, Giannone G, Le Clainche C, Helfer E, Sengupta K. Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls. International Journal of Molecular Sciences. 2021; 22(12):6335. https://doi.org/10.3390/ijms22126335

Chicago/Turabian Style

Souissi, Mariem, Julien Pernier, Olivier Rossier, Gregory Giannone, Christophe Le Clainche, Emmanuèle Helfer, and Kheya Sengupta. 2021. "Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls" International Journal of Molecular Sciences 22, no. 12: 6335. https://doi.org/10.3390/ijms22126335

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