Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to Verticillium wilt
Abstract
:1. Introduction
2. Results
2.1. Identification of NOT2_3_5 Domain Protein Family in Cotton
2.2. Phylogenetic and Conserved Amino Residue Analysis of NOT2_3_5 Domain Proteins
2.3. Chromosome Location and Gene Collinearity Analysis
2.4. Cis-Regulatory Elements Analysis of NOT 2_3_5 Promoter Regions
2.5. Prediction of MiRNAs Targeting GhNOT2_3_5 Domain Protein Genes
2.6. Expression Patterns of NOT2_3_5 Genes in Different Tissues and Responding to Stresses and Phytohormones
2.7. Silencing of GbNOT2_3_5 Genes Reduced Verticillium Wilt Resistance in Cotton
3. Discussion
3.1. Evolution and Duplication of NOT2_3_5 Genes
3.2. Relationship between MiRNA and NOT2_3_5 Genes
3.3. Differences between NOT2_3_5 Domain Proteins from Two Classes
3.4. Expression Patterns and Putative Disease Resistant Functions of NOT2_3_5 Domain Protein Genes
4. Materials and Methods
4.1. Identification of the NOT2_3_5 Domain Protein Family
4.2. Construction of Phylogenetic Tree and Chromosome Map
4.3. Gene Collinearity and Structural Analysis
4.4. Target Prediction between MiRNAs and GhNOT2_3_5 Members
4.5. Prediction of Upstream cis-Elements
4.6. Expression Pattern Analysis
4.7. Vector Construction and Development of Virus-Induced Gene Silencing (VIGS) in Cotton
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
NOT | Negative on TATA-less |
CCR4-NOT | Carbon Catabolite Repression 4-NOT |
SA | Salicylic Acid |
ET | Ethylene |
MeJA | Methyl Jasmonate |
VIGS | Virus-Induced Gene Silencing |
pI | isoelectric point |
MW | molecular weight |
Dpa | Days Post Anthesis |
TRV | Tobacco Rattle Virus |
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Zhao, P.; Qin, T.; Chen, W.; Sang, X.; Zhao, Y.; Wang, H. Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to Verticillium wilt. Int. J. Mol. Sci. 2021, 22, 5634. https://doi.org/10.3390/ijms22115634
Zhao P, Qin T, Chen W, Sang X, Zhao Y, Wang H. Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to Verticillium wilt. International Journal of Molecular Sciences. 2021; 22(11):5634. https://doi.org/10.3390/ijms22115634
Chicago/Turabian StyleZhao, Pei, Tengfei Qin, Wei Chen, Xiaohui Sang, Yunlei Zhao, and Hongmei Wang. 2021. "Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance to Verticillium wilt" International Journal of Molecular Sciences 22, no. 11: 5634. https://doi.org/10.3390/ijms22115634