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Differences in Stability of Viral and Viral-Cellular Fusion Transcripts in HPV-Induced Cervical Cancers
Open AccessArticle

HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

1
Department of Virology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Krakow, Poland
2
Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa Street, 30-387 Krakow, Poland
3
Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 14 Bobrzynskiego Street, 30-348 Krakow, Poland
4
Centre of Microbiological Research and Autovaccines, 17 Slawkowska Street, 31-016 Krakow, Poland
5
National Research Institute of Oncology, Krakow Branch, Clinic of Radiotherapy, 11 Garncarska Street, 31-115 Krakow, Poland
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2667; https://doi.org/10.3390/ijms21082667
Received: 20 March 2020 / Revised: 8 April 2020 / Accepted: 8 April 2020 / Published: 11 April 2020
(This article belongs to the Special Issue Molecular Research on Cervical Cancer)
Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV, IIa/HPV, IIa/HPV, LSIL/HPV, LSIL/HPV+, HSIL/HPV, HSIL/HPV+ and cancer cells (SCC/HPV+). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics. View Full-Text
Keywords: human papillomavirus; glycogen; cervical cancer; cervical dysplasia; Raman microscopy; glycogenolysis human papillomavirus; glycogen; cervical cancer; cervical dysplasia; Raman microscopy; glycogenolysis
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MDPI and ACS Style

Sitarz, K.; Czamara, K.; Bialecka, J.; Klimek, M.; Zawilinska, B.; Szostek, S.; Kaczor, A. HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution. Int. J. Mol. Sci. 2020, 21, 2667.

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