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Open AccessArticle

Quantitative Proteomic Analyses Identify STO/BBX24 -Related Proteins Induced by UV-B

1
Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou 510631, China
2
Hunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha 410128, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(7), 2496; https://doi.org/10.3390/ijms21072496
Received: 26 February 2020 / Revised: 30 March 2020 / Accepted: 31 March 2020 / Published: 3 April 2020
(This article belongs to the Section Molecular Plant Sciences)
Plants use solar radiation for photosynthesis and are inevitably exposed to UV-B. To adapt to UV-B radiation, plants have evolved a sophisticated strategy, but the mechanism is not well understood. We have previously reported that STO (salt tolerance)/BBX24 is a negative regulator of UV-B-induced photomorphogenesis. However, there is limited knowledge of the regulatory network of STO in UV-B signaling. Here, we report the identification of proteins differentially expressed in the wild type (WT) and sto mutant after UV-B radiation by iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomic analysis to explore differential proteins that depend on STO and UV-B signaling. A total of 8212 proteins were successfully identified, 221 of them were STO-dependent proteins in UV-B irradiated plants. The abundances of STO-dependent PSB and LHC (light-harvesting complex) proteins in sto mutants decreased under UV-B radiation, suggesting that STO is necessary to maintain the normal accumulation of photosynthetic system complex under UV-B radiation to facilitate photosynthesis photon capture. The abundance of phenylalanine lyase-1 (PAL1), chalcone synthetase (CHS), and flavonoid synthetase (FLS) increased significantly after UV-B irradiation, suggesting that the accumulation of flavonoids do not require STO, but UV-B is needed. Under UV-B radiation, STO stabilizes the structure of antenna protein complex by maintaining the accumulation of PSBs and LHCs, thereby enhancing the non-photochemical quenching (NPQ) ability, releasing extra energy, protecting photosynthesis, and ultimately promoting the elongation of hypocotyl. The accumulation of flavonoid synthesis key proteins is independent of STO under UV-B radiation. Overall, our results provide a comprehensive regulatory network of STO in UV-B signaling. View Full-Text
Keywords: UV-B irradiation; STO (salt tolerance)/BBX24; photosynthesis; antenna protein; flavanol; anthocyanin; NPQ (non-photochemical quenching) UV-B irradiation; STO (salt tolerance)/BBX24; photosynthesis; antenna protein; flavanol; anthocyanin; NPQ (non-photochemical quenching)
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Lyu, G.; Li, D.; Xiong, H.; Xiao, L.; Tong, J.; Ning, C.; Wang, P.; Li, S. Quantitative Proteomic Analyses Identify STO/BBX24 -Related Proteins Induced by UV-B. Int. J. Mol. Sci. 2020, 21, 2496.

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