Biological Activities and Molecular Docking of Brassinosteroids 24-Norcholane Type Analogs
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry
2.2. Bioactivity in the Rice Lamina Inclination Assay of Brassinosteroids Analogs.
2.3. Molecular Docking
3. Materials and Methods
3.1. Biological Activity
3.1.1. Rice Lamina Inclination Test
3.1.2. Root Elongation of A. Thaliana
3.2. Molecular Docking
3.2.1. Ligand/Molecular Target Selection and Preparation
3.2.2. Docking Procedure and Analysis of Protein–Ligand Complexes
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BL | Brassinolide |
BRs | Brassinosteroids |
CS | Castasterone |
RLIT | Rice leaf inclination test |
BSIB | Bean second node bioassay |
SAR | Structure-activity Relationships |
BRI1LRR | BL receptor with extracellular leucine-rich domain |
BAK1 | BRI1-associated kinase 1 |
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Bending Angle Between Laminae and Sheaths (Degrees ± Standard Error) | |||
---|---|---|---|
Compounds | 1 × 10−8 M | 1 × 10−7 M | 1 × 10−6 M |
BL (C+) | 31 ± 11 | 41 ± 4.5 | 70 ± 7.6 |
3 | 35 ± 3 | 60 ± 3 * | 62 ± 12 |
4 | 45 ± 9.5 * | 31 ± 5 | 24 ± 5.8 * |
5 | 5 ± 2.7 * | 13 ± 1.7 * | 28 ± 2.5 * |
6 | 49 ± 5 * | 60 ± 5 * | 78 ± 15 * |
7 | 28 ± 3 | 64 ± 3 * | 70 ± 10 |
8 | 24 ± 2.5 | 34 ± 2.5 | 35 ± 2.9 * |
9 | 34 ± 2.2 | 45 ± 2.7 | 53 ± 6.3 * |
3/4 (1.0:0.24) | 26 ± 2.5 | 63 ± 2.9 * | 66 ± 6.3 |
3/4 (1.0:0.9) | 35 ± 3.8 | 43 ± 5.0 | 38 ± 5 |
Control (C-) | 7 ± 5 |
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Díaz, K.; Espinoza, L.; Carvajal, R.; Conde-González, M.; Niebla, V.; Olea, A.F.; Coll, Y. Biological Activities and Molecular Docking of Brassinosteroids 24-Norcholane Type Analogs. Int. J. Mol. Sci. 2020, 21, 1832. https://doi.org/10.3390/ijms21051832
Díaz K, Espinoza L, Carvajal R, Conde-González M, Niebla V, Olea AF, Coll Y. Biological Activities and Molecular Docking of Brassinosteroids 24-Norcholane Type Analogs. International Journal of Molecular Sciences. 2020; 21(5):1832. https://doi.org/10.3390/ijms21051832
Chicago/Turabian StyleDíaz, Katy, Luis Espinoza, Rodrigo Carvajal, Marcos Conde-González, Vladimir Niebla, Andrés F. Olea, and Yamilet Coll. 2020. "Biological Activities and Molecular Docking of Brassinosteroids 24-Norcholane Type Analogs" International Journal of Molecular Sciences 21, no. 5: 1832. https://doi.org/10.3390/ijms21051832