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Bisphenol S Impaired Human Granulosa Cell Steroidogenesis in Vitro

PRC, INRAE, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France
Service de Chirurgie pédiatrique viscérale, urologique, plastique et brûlés, CHRU de Tours, 37000 Tours, France
Therapeutic Innovations and Resistance (INTHERES), Université de Toulouse, INRAE, ENVT, 31076 Toulouse, France
Service de Médecine et Biologie de la Reproduction, CHRU de Tours, 37000 Tours, France
Author to whom correspondence should be addressed.
This authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(5), 1821;
Received: 13 February 2020 / Revised: 3 March 2020 / Accepted: 5 March 2020 / Published: 6 March 2020
(This article belongs to the Special Issue Advances in the Research of Endocrine Disrupting Chemicals 2.0)
Bisphenol S (BPS) is a structural analog of the endocrine disruptor bisphenol A (BPA); it is the main BPA replacement in the plastics industry. Previous studies have shown that BPA and BPS exhibit similar effects on reproduction in fish and rodent species. BPS reportedly alters steroidogenesis in bovine granulosa cells. Luteinised granulosa cells collected from 59 women who were undergoing an in vitro fertilization procedure were cultured for 48 h in the presence or absence of BPS (10 nM, 100 nM, 1 µM, 10 µM or 50 µM). BPS exposure was investigated by assessing follicular fluids from these 59 women for their BPS content. Culture medium, cells, total messenger RNA (mRNA) and total protein extracted from the luteinised granulosa cells were examined for oestradiol and progesterone secretion, cellular proliferation, viability, gene expression, steroidogenic enzyme expression and cell signaling. BPS was measured in follicular fluids using mass spectrometry. Exposure of granulosa cells to 10 or 50 µM BPS for 48 h induced a 16% (p = 0.0059) and 64% (p < 0.0001) decrease, respectively, in progesterone secretion; 50 µM BPS decreased oestradiol secretion by 46% (p < 0.0001). Ten µM BPS also tended to reduce CYP11A1 protein expression by 37% (p = 0.0947) without affecting HSD3B1 and CYP19A1 expression. Fifty µM BPS increased ERRγ expression. Environmental levels of BPS (nanomolar range) did not induce changes in steroidogenesis in human granulosa cells. The effects of BPS were observed after only 48 h of BPS exposure. These acute effects might be similar to chronic effects of physiological BPS levels. View Full-Text
Keywords: endocrine disruptors; Bisphenol S; women; granulosa cells; steroidogenesis; proliferation endocrine disruptors; Bisphenol S; women; granulosa cells; steroidogenesis; proliferation
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MDPI and ACS Style

Amar, S.; Binet, A.; Téteau, O.; Desmarchais, A.; Papillier, P.; Lacroix, M.Z.; Maillard, V.; Guérif, F.; Elis, S. Bisphenol S Impaired Human Granulosa Cell Steroidogenesis in Vitro. Int. J. Mol. Sci. 2020, 21, 1821.

AMA Style

Amar S, Binet A, Téteau O, Desmarchais A, Papillier P, Lacroix MZ, Maillard V, Guérif F, Elis S. Bisphenol S Impaired Human Granulosa Cell Steroidogenesis in Vitro. International Journal of Molecular Sciences. 2020; 21(5):1821.

Chicago/Turabian Style

Amar, Sarah, Aurélien Binet, Ophélie Téteau, Alice Desmarchais, Pascal Papillier, Marlène Z. Lacroix, Virginie Maillard, Fabrice Guérif, and Sebastien Elis. 2020. "Bisphenol S Impaired Human Granulosa Cell Steroidogenesis in Vitro" International Journal of Molecular Sciences 21, no. 5: 1821.

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