Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)
Abstract
:1. Introduction
2. Results
2.1. Identification of saT1R Genes
2.2. Phylogenetic Analysis of saT1Rs and saGαi Genes
2.3. Validation of pGL3-NFAT-luc Reporter Constructs for Intracellular Ca2+ Quantification in Transiently Transfected HEK293 Cells
2.4. Validation of Stable pGL3-NFAT-Luc-HEK293 Cell Lines
2.5. In Vitro Characterization of saT1: Responses to L-AAs
2.6. In Vitro Characterization of saT1R: Responses to Natural Sugars
2.7. Pharmacological Responses to Proline in the Presence of saGαi1-2
3. Discussion
4. Materials and Methods
4.1. In Silico Identification and Molecular Cloning of saT1Rs and saGαi Genes
4.2. Phylogenetic Analyses
4.3. Generation of Stable pGL3-NFAT-Luc HEK293 Cell Lines
4.4. Transient Transfections and Stimulation Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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T1R1/R3 | EC50 | T1R2a/R3 | EC50 | T1R2b/R3 | EC50 |
---|---|---|---|---|---|
AAs | (sensitivity)↓ | AAs | (sensitivity)↓ | AAs | (sensitivity)↓ |
Leu | 1,61 × 10−4 | Val | 1,24 × 10−3 | Glu | 1,68 × 10−6 |
Ser | 9,47 × 10−4 | Gly | 1,67 × 10−3 | Ser | 9,94 × 10−5 |
Ala | 1,02 × 10−3 | Leu | 6,03 × 10−3 | Leu | 1,88 × 10−3 |
Pro | 1,16 × 10−3 | Pro | 7,29 × 10−3 | Pro | 2,10 × 10−3 |
Gly | 1,97 × 10−3 | Gln | 9,62 × 10−3 | Gly | 4,88 × 10−3 |
Met | 3,77 × 10−3 | Thr | 1,96 × 10−2 | Ile | 9,55 × 10−2 |
Ile | 8,06 × 10−2 | Met | 4,76 × 10−2 | Met | 1,49 × 10−1 |
Ile | 1,01 × 10−1 |
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Angotzi, A.R.; Puchol, S.; Cerdá-Reverter, J.M.; Morais, S. Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata). Int. J. Mol. Sci. 2020, 21, 7732. https://doi.org/10.3390/ijms21207732
Angotzi AR, Puchol S, Cerdá-Reverter JM, Morais S. Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata). International Journal of Molecular Sciences. 2020; 21(20):7732. https://doi.org/10.3390/ijms21207732
Chicago/Turabian StyleAngotzi, Anna Rita, Sara Puchol, Jose M. Cerdá-Reverter, and Sofia Morais. 2020. "Insights into the Function and Evolution of Taste 1 Receptor Gene Family in the Carnivore Fish Gilthead Seabream (Sparus aurata)" International Journal of Molecular Sciences 21, no. 20: 7732. https://doi.org/10.3390/ijms21207732