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Open AccessArticle

The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides

1
Federal Research and Clinical Center of Physical Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia
2
Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow 141701, Russia
3
Department of biochemistry, Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia
4
Sechenov First Moscow State Medical University Sechenov University, Moscow 119991, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7141; https://doi.org/10.3390/ijms21197141
Received: 3 September 2020 / Revised: 24 September 2020 / Accepted: 25 September 2020 / Published: 27 September 2020
(This article belongs to the Special Issue Creation of New Antimicrobial Peptides)
Antimicrobial peptides (AMPs) are considered a promising new class of anti-infectious agents. This study reports new antimicrobial peptides derived from the Hirudo medicinalis microbiome identified by a computational analysis method applied to the H. medicinalis metagenome. The identified AMPs possess a strong antimicrobial activity against Gram-positive and Gram-negative bacteria (MIC range: 5.3 to 22.4 μM), including Staphylococcus haemolyticus, an opportunistic coagulase–negative pathogen. The secondary structure analysis of peptides via CD spectroscopy showed that all the AMPs except pept_352 have mostly disordered structures that do not change under different conditions. For peptide pept_352, the α–helical content increases in the membrane environment. The examination of the mechanism of action of peptides suggests that peptide pept_352 exhibits a direct membranolytic activity. Furthermore, the cytotoxicity assay demonstrated that the nontoxic peptide pept_1545 is a promising candidate for drug development. Overall, the analysis method implemented in the study may serve as an effective tool for the identification of new AMPs. View Full-Text
Keywords: microbiome; metagenome; Hirudo medicinalis; antimicrobial peptides; bioinformatic analysis; secondary structure; circular dichroism; α–helix microbiome; metagenome; Hirudo medicinalis; antimicrobial peptides; bioinformatic analysis; secondary structure; circular dichroism; α–helix
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MDPI and ACS Style

Grafskaia, E.; Pavlova, E.; Babenko, V.V.; Latsis, I.; Malakhova, M.; Lavrenova, V.; Bashkirov, P.; Belousov, D.; Klinov, D.; Lazarev, V. The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides. Int. J. Mol. Sci. 2020, 21, 7141. https://doi.org/10.3390/ijms21197141

AMA Style

Grafskaia E, Pavlova E, Babenko VV, Latsis I, Malakhova M, Lavrenova V, Bashkirov P, Belousov D, Klinov D, Lazarev V. The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides. International Journal of Molecular Sciences. 2020; 21(19):7141. https://doi.org/10.3390/ijms21197141

Chicago/Turabian Style

Grafskaia, Ekaterina; Pavlova, Elizaveta; Babenko, Vladislav V.; Latsis, Ivan; Malakhova, Maja; Lavrenova, Victoria; Bashkirov, Pavel; Belousov, Dmitrii; Klinov, Dmitry; Lazarev, Vassili. 2020. "The Hirudo Medicinalis Microbiome Is a Source of New Antimicrobial Peptides" Int. J. Mol. Sci. 21, no. 19: 7141. https://doi.org/10.3390/ijms21197141

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