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Open AccessArticle

Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

1
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
College of Life Sciences, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Shijingshan District, Beijing 100049, China
3
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou 510650, China
4
Independent Researcher, P.O. Box 7, Miki-Cho Post Office, Ikenobe 3011-2, Kagawa-ken 761-0799, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(19), 7005; https://doi.org/10.3390/ijms21197005
Received: 1 September 2020 / Revised: 16 September 2020 / Accepted: 21 September 2020 / Published: 23 September 2020
(This article belongs to the Special Issue Orchid Biochemistry 2.0)
Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids. View Full-Text
Keywords: floral volatiles; geraniol; MEP pathway; orchids; terpene synthase floral volatiles; geraniol; MEP pathway; orchids; terpene synthase
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MDPI and ACS Style

Zhao, C.; Yu, Z.; Silva, J.A.T.d.; He, C.; Wang, H.; Si, C.; Zhang, M.; Zeng, D.; Duan, J. Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation. Int. J. Mol. Sci. 2020, 21, 7005.

AMA Style

Zhao C, Yu Z, Silva JATd, He C, Wang H, Si C, Zhang M, Zeng D, Duan J. Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation. International Journal of Molecular Sciences. 2020; 21(19):7005.

Chicago/Turabian Style

Zhao, Conghui; Yu, Zhenming; Silva, Jaime A.T.d.; He, Chunmei; Wang, Haobin; Si, Can; Zhang, Mingze; Zeng, Danqi; Duan, Jun. 2020. "Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation" Int. J. Mol. Sci. 21, no. 19: 7005.

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