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Open AccessArticle

Development of a Rat Model for Glioma-Related Epilepsy

1
4Brain, Department of Head and Skin, Ghent University, 9000 Ghent, East Flanders, Belgium
2
Department of Pharmaceutical Analysis, Ghent University, 9000 Ghent, East Flanders, Belgium
3
Early Solutions, Neuroscience Therapeutic Area, UCB Pharma, 1420 Braine-l’Alleud, Brabant Wallon, Belgium
4
Department of Electronics and information systems, Ghent University Hospital, 9000 Ghent, East Flanders, Belgium
5
Department of Diagnostic Sciences, Ghent University Hospital, 9000 Ghent, East Flanders, Belgium
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 6999; https://doi.org/10.3390/ijms21196999
Received: 30 July 2020 / Revised: 18 September 2020 / Accepted: 21 September 2020 / Published: 23 September 2020
Seizures are common in patients with high-grade gliomas (30–60%) and approximately 15–30% of glioblastoma (GB) patients develop drug-resistant epilepsy. Reliable animal models are needed to develop adequate treatments for glioma-related epilepsy. Therefore, fifteen rats were inoculated with F98 GB cells (GB group) and four rats with vehicle only (control group) in the right entorhinal cortex. MRI was performed to visualize tumor presence. A subset of seven GB and two control rats were implanted with recording electrodes to determine the occurrence of epileptic seizures with video-EEG recording over multiple days. In a subset of rats, tumor size and expression of tumor markers were investigated with histology or mRNA in situ hybridization. Tumors were visible on MRI six days post-inoculation. Time-dependent changes in tumor morphology and size were visible on MRI. Epileptic seizures were detected in all GB rats monitored with video-EEG. Twenty-one days after inoculation, rats were euthanized based on signs of discomfort and pain. This study describes, for the first time, reproducible tumor growth and spontaneous seizures upon inoculation of F98 cells in the rat entorhinal cortex. The development of this new model of GB-related epilepsy may be valuable to design new therapies against tumor growth and associated epileptic seizures. View Full-Text
Keywords: glioma; seizures; high-grade glioma rat model; glioma-related epilepsy; video-EEG monitoring glioma; seizures; high-grade glioma rat model; glioma-related epilepsy; video-EEG monitoring
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Bouckaert, C.; Germonpré, C.; Verhoeven, J.; Chong, S.-A.; Jacquin, L.; Mairet-Coello, G.; André, V.M.; Leclercq, K.; Vanhove, C.; De Vos, F.; Van den Broecke, C.; Goethals, I.; Descamps, B.; Donche, S.; Carrette, E.; Wadman, W.; Boon, P.; Vonck, K.; Raedt, R. Development of a Rat Model for Glioma-Related Epilepsy. Int. J. Mol. Sci. 2020, 21, 6999.

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