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Article

The Salt Sensitivity Induced by Disruption of Cell Wall-Associated Kinase 1 (SlWAK1) Tomato Gene Is Linked to Altered Osmotic and Metabolic Homeostasis

1
Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100 Murcia, Spain
2
Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Universidad de Almería, 04120 Almería, Spain
3
Institut de Biologie de l’École Normale Supérieure (IBENS), Paris Sciences et Lettres Research University, F-75005 Paris, France
4
BASF Vegetable Seeds, Napoleonsweg 152, 6083AB Nunhem, The Netherlands
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Present addresses: Department of Molecular Biology and Biochemistry, Instituto de Hortofruticultura Subtropical y Mediterránea, University of Malaga-CSIC, 29071 Malaga, Spain.
§
Present addresses: Plateforme EPItrans, Institute of Plant Sciences (IPS2), 630 Rue Noetzlin Bâtiment, 91190 Gif-sur-Yvette, France.
Int. J. Mol. Sci. 2020, 21(17), 6308; https://doi.org/10.3390/ijms21176308
Received: 29 July 2020 / Revised: 24 August 2020 / Accepted: 28 August 2020 / Published: 31 August 2020
Tomato cell wall-associated kinase 1 (SlWAK1) has only been studied in biotic stress response and hence its function in abiotic stress remains unknown. In a screening under salinity of an insertional mutant collection of tomato (Solanum lycopersicum L.), a mutant exhibiting lower degree of leaf chlorosis than wild type (WT) together with reduced leaf Na+ accumulation was selected. Genetic analysis of the mutation revealed that a single T-DNA insertion in the SlWAK1 gene was responsible of the mutant phenotype. Slwak1 null mutant reduced its shoot growth compared with WT, despite its improved Na+ homeostasis. SlWAK1 disruption affected osmotic homeostasis, as leaf water content was lower in mutant than in WT under salt stress. In addition, Slwak1 altered the source-sink balance under salinity, by increasing sucrose content in roots. Finally, a significant fruit yield reduction was found in Slwak1 vs. WT under long-term salt stress, mainly due to lower fruit weight. Our results show that disruption of SlWAK1 induces a higher sucrose transport from source leaf to sink root, negatively affecting fruit, the main sink at adult stage. View Full-Text
Keywords: Solanum lycopersicum; insertional mutant; osmotic stress; ion stress; metabolites; fruit yield Solanum lycopersicum; insertional mutant; osmotic stress; ion stress; metabolites; fruit yield
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MDPI and ACS Style

Meco, V.; Egea, I.; Ortíz-Atienza, A.; Drevensek, S.; Esch, E.; Yuste-Lisbona, F.J.; Barneche, F.; Vriezen, W.; Bolarin, M.C.; Lozano, R.; Flores, F.B. The Salt Sensitivity Induced by Disruption of Cell Wall-Associated Kinase 1 (SlWAK1) Tomato Gene Is Linked to Altered Osmotic and Metabolic Homeostasis. Int. J. Mol. Sci. 2020, 21, 6308. https://doi.org/10.3390/ijms21176308

AMA Style

Meco V, Egea I, Ortíz-Atienza A, Drevensek S, Esch E, Yuste-Lisbona FJ, Barneche F, Vriezen W, Bolarin MC, Lozano R, Flores FB. The Salt Sensitivity Induced by Disruption of Cell Wall-Associated Kinase 1 (SlWAK1) Tomato Gene Is Linked to Altered Osmotic and Metabolic Homeostasis. International Journal of Molecular Sciences. 2020; 21(17):6308. https://doi.org/10.3390/ijms21176308

Chicago/Turabian Style

Meco, Victoriano, Isabel Egea, Ana Ortíz-Atienza, Stéphanie Drevensek, Elisabeth Esch, Fernando J. Yuste-Lisbona, Fredy Barneche, Wim Vriezen, María C. Bolarin, Rafael Lozano, and Francisco B. Flores 2020. "The Salt Sensitivity Induced by Disruption of Cell Wall-Associated Kinase 1 (SlWAK1) Tomato Gene Is Linked to Altered Osmotic and Metabolic Homeostasis" International Journal of Molecular Sciences 21, no. 17: 6308. https://doi.org/10.3390/ijms21176308

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