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Open AccessArticle

Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots

1
Genomics Division, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2
Plant Systems Engineering Research Center, KRIBB, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(16), 5721; https://doi.org/10.3390/ijms21165721
Received: 9 July 2020 / Revised: 3 August 2020 / Accepted: 5 August 2020 / Published: 10 August 2020
(This article belongs to the Collection Plant Health and Food Security)
Glucoraphasatin (GRH) is a specific aliphatic glucosinolate (GSL) that is only abundant in radish (Raphanus sativus L.). The gene expression regulating GRH biosynthesis in radish is still poorly understood. We employed a total of 59 radish accessions to analyze GSL profiles and showed that GRH was specific and predominant among the aliphatic GSLs in radish roots. We selected five accessions roots with high, moderate and low GSL biosynthesis, respectively, to conduct a comparative transcriptome analysis and the qRT-PCR of the biosynthesis genes for aliphatic GSLs. In this study, among all the accessions tested, roots with the accession RA157-74 had a high GRH content and showed a significant expression of the aliphatic GSL biosynthesis genes. We defined the genes involved in the GRH biosynthesis process and found that they were regulated by a transcription factor (RSG00789) at the MYB29 locus in radish roots. We found 13 aliphatic GSL biosynthesis genes regulated by the RSG00789 gene in the GRH biosynthesis pathway. View Full-Text
Keywords: glucoraphasatin; glucosinolates; MYB transcription factors; radish transcriptome; GRS1 glucoraphasatin; glucosinolates; MYB transcription factors; radish transcriptome; GRS1
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MDPI and ACS Style

Kang, J.-N.; Won, S.Y.; Seo, M.-S.; Lee, J.; Lee, S.M.; Kwon, S.-J.; Kim, J.S. Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots. Int. J. Mol. Sci. 2020, 21, 5721. https://doi.org/10.3390/ijms21165721

AMA Style

Kang J-N, Won SY, Seo M-S, Lee J, Lee SM, Kwon S-J, Kim JS. Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots. International Journal of Molecular Sciences. 2020; 21(16):5721. https://doi.org/10.3390/ijms21165721

Chicago/Turabian Style

Kang, Ji-Nam; Won, So Y.; Seo, Mi-Suk; Lee, Jeongyeo; Lee, Si M.; Kwon, Soo-Jin; Kim, Jung S. 2020. "Induction of Glucoraphasatin Biosynthesis Genes by MYB29 in Radish (Raphanus sativus L.) Roots" Int. J. Mol. Sci. 21, no. 16: 5721. https://doi.org/10.3390/ijms21165721

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