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Article

Protein Kinase CK2 Controls CaV2.1-Dependent Calcium Currents and Insulin Release in Pancreatic β-cells

1
Department of Medical Biochemistry and Molecular Biology, Saarland University, Kirrberger Str., bldg. 44, D-66424 Homburg, Germany
2
Department of Experimental and Clinical Pharmacology and Toxicology, Saarland University Kirrberger Str., bldg. 45-46, D-66424 Homburg, Germany
3
Institute for Clinical & Experimental Surgery, Saarland University Kirrberger Str., bldg. 65, D-66424 Homburg, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4668; https://doi.org/10.3390/ijms21134668
Received: 14 May 2020 / Revised: 18 June 2020 / Accepted: 26 June 2020 / Published: 30 June 2020
(This article belongs to the Section Molecular Endocrinology and Metabolism)
The regulation of insulin biosynthesis and secretion in pancreatic β-cells is essential for glucose homeostasis in humans. Previous findings point to the highly conserved, ubiquitously expressed serine/threonine kinase CK2 as having a negative regulatory impact on this regulation. In the cell culture model of rat pancreatic β-cells INS-1, insulin secretion is enhanced after CK2 inhibition. This enhancement is preceded by a rise in the cytosolic Ca2+ concentration. Here, we identified the serine residues S2362 and S2364 of the voltage-dependent calcium channel CaV2.1 as targets of CK2 phosphorylation. Furthermore, co-immunoprecipitation experiments revealed that CaV2.1 binds to CK2 in vitro and in vivo. CaV2.1 knockdown experiments showed that the increase in the intracellular Ca2+ concentration, followed by an enhanced insulin secretion upon CK2 inhibition, is due to a Ca2+ influx through CaV2.1 channels. In summary, our results point to a modulating role of CK2 in the CaV2.1-mediated exocytosis of insulin. View Full-Text
Keywords: CK2; INS-1; CX-4945; CaV2.1; insulin secretion CK2; INS-1; CX-4945; CaV2.1; insulin secretion
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MDPI and ACS Style

Scheuer, R.; Philipp, S.E.; Becker, A.; Nalbach, L.; Ampofo, E.; Montenarh, M.; Götz, C. Protein Kinase CK2 Controls CaV2.1-Dependent Calcium Currents and Insulin Release in Pancreatic β-cells. Int. J. Mol. Sci. 2020, 21, 4668. https://doi.org/10.3390/ijms21134668

AMA Style

Scheuer R, Philipp SE, Becker A, Nalbach L, Ampofo E, Montenarh M, Götz C. Protein Kinase CK2 Controls CaV2.1-Dependent Calcium Currents and Insulin Release in Pancreatic β-cells. International Journal of Molecular Sciences. 2020; 21(13):4668. https://doi.org/10.3390/ijms21134668

Chicago/Turabian Style

Scheuer, Rebecca; Philipp, Stephan E.; Becker, Alexander; Nalbach, Lisa; Ampofo, Emmanuel; Montenarh, Mathias; Götz, Claudia. 2020. "Protein Kinase CK2 Controls CaV2.1-Dependent Calcium Currents and Insulin Release in Pancreatic β-cells" Int. J. Mol. Sci. 21, no. 13: 4668. https://doi.org/10.3390/ijms21134668

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