Next Article in Journal
Cancer Immunotherapeutic Potential of NKTT320, a Novel, Invariant, Natural Killer T Cell-Activating, Humanized Monoclonal Antibody
Previous Article in Journal
Artificial Sporulation Induction (ASI) by kinA Overexpression Affects the Proteomes and Properties of Bacillus subtilis Spores
Open AccessArticle

Comparative Transcriptome Analysis of Two Contrasting Soybean Varieties in Response to Aluminum Toxicity

1
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China
2
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4316; https://doi.org/10.3390/ijms21124316
Received: 1 May 2020 / Revised: 9 June 2020 / Accepted: 12 June 2020 / Published: 17 June 2020
(This article belongs to the Section Molecular Plant Sciences)
Aluminum (Al) toxicity is a major factor limiting crop productivity on acid soils. Soybean (Glycine max) is an important oil crop and there is great variation in Al tolerance in soybean germplasms. However, only a few Al-tolerance genes have been reported in soybean. Therefore, the purpose of this study was to identify candidate Al tolerance genes by comparative transcriptome analysis of two contrasting soybean varieties in response to Al stress. Two soybean varieties, M90-24 (M) and Pella (P), which showed significant difference in Al tolerance, were used for RNA-seq analysis. We identified a total of 354 Al-tolerance related genes, which showed up-regulated expression by Al in the Al-tolerant soybean variety M and higher transcript levels in M than P under Al stress. These genes were enriched in the Gene Ontology (GO) terms of cellular glucan metabolic process and regulation of transcription. Five out of 11 genes in the enriched GO term of cellular glucan metabolic process encode cellulose synthases, and one cellulose synthase gene (Glyma.02G205800) was identified as the key hub gene by co-expression network analysis. Furthermore, treatment of soybean roots with a cellulose biosynthesis inhibitor decreased the Al tolerance, indicating an important role of cellulose production in soybean tolerance to Al toxicity. This study provides a list of candidate genes for further investigation on Al tolerance mechanisms in soybean. View Full-Text
Keywords: aluminum; cellulose; gene ontology (GO) enrichment; RNA-seq; soybean aluminum; cellulose; gene ontology (GO) enrichment; RNA-seq; soybean
Show Figures

Figure 1

MDPI and ACS Style

Zhao, L.; Cui, J.; Cai, Y.; Yang, S.; Liu, J.; Wang, W.; Gai, J.; Hu, Z.; Li, Y. Comparative Transcriptome Analysis of Two Contrasting Soybean Varieties in Response to Aluminum Toxicity. Int. J. Mol. Sci. 2020, 21, 4316.

AMA Style

Zhao L, Cui J, Cai Y, Yang S, Liu J, Wang W, Gai J, Hu Z, Li Y. Comparative Transcriptome Analysis of Two Contrasting Soybean Varieties in Response to Aluminum Toxicity. International Journal of Molecular Sciences. 2020; 21(12):4316.

Chicago/Turabian Style

Zhao, Lijuan; Cui, Jingjing; Cai, Yuanyuan; Yang, Songnan; Liu, Juge; Wang, Wei; Gai, Junyi; Hu, Zhubing; Li, Yan. 2020. "Comparative Transcriptome Analysis of Two Contrasting Soybean Varieties in Response to Aluminum Toxicity" Int. J. Mol. Sci. 21, no. 12: 4316.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop