Next Article in Journal
Fibroblasts as a Biological Marker for Curative Resection in Pancreatic Ductal Adenocarcinoma
Next Article in Special Issue
MnO2 Heterostructure on Carbon Nanotubes as Cathode Material for Aqueous Zinc-Ion Batteries
Previous Article in Journal
Cell Distribution within Yeast Colonies and Colony Biofilms: How Structure Develops
Previous Article in Special Issue
Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco
Article

Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension

1
Soft Matter Physics Division and Biotechnology & Biomedical Group, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Linnéstr. 5, 04103 Leipzig, Germany
2
Paul Flechsig Institute for Brain Research, Leipzig University, Liebigstr. 19, 04103 Leipzig, Germany
3
Division of Surface Physics, Department of Physics and Earth Sciences, Leipzig University and Leibniz Institute of Surface Engineering (IOM), Permoser Str. 15, 04318 Leipzig, Germany
4
Institute of Food Hygiene, Faculty of Veterinary Medicine, Leipzig University, Augustusplatz 10, 04109 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Present address: Department of Pediatrics, Weill Cornell Medical College, York Ave, NY 1300, USA.
Present address: Department of Safety and Quality of Meat, Federal Research Institute of Nutrition and Food, Max Rubner-Institut (MRI), E.-C.-Baumann-Str. 20, 95326 Kulmbach, Germany.
Int. J. Mol. Sci. 2020, 21(11), 3889; https://doi.org/10.3390/ijms21113889
Received: 11 May 2020 / Revised: 25 May 2020 / Accepted: 25 May 2020 / Published: 29 May 2020
(This article belongs to the Special Issue Nano-Materials and Methods 2.0)
Numerous eye diseases are linked to biomechanical dysfunction of the retina. However, the underlying forces are almost impossible to quantify experimentally. Here, we show how biomechanical properties of adult neuronal tissues such as porcine retinae can be investigated under tension in a home-built tissue stretcher composed of nanostructured TiO2 scaffolds coupled to a self-designed force sensor. The employed TiO2 nanotube scaffolds allow for organotypic long-term preservation of adult tissues ex vivo and support strong tissue adhesion without the application of glues, a prerequisite for tissue investigations under tension. In combination with finite element calculations we found that the deformation behavior is highly dependent on the displacement rate which results in Young’s moduli of (760–1270) Pa. Image analysis revealed that the elastic regime is characterized by a reversible shear deformation of retinal layers. For larger deformations, tissue destruction and sliding of retinal layers occurred with an equilibration between slip and stick at the interface of ruptured layers, resulting in a constant force during stretching. Since our study demonstrates how porcine eyes collected from slaughterhouses can be employed for ex vivo experiments, our study also offers new perspectives to investigate tissue biomechanics without excessive animal experiments. View Full-Text
Keywords: retina; nanostructured scaffolds; tissue mechanics; nanotubes; tissue stretcher; tissue elasticity; porcine eyes retina; nanostructured scaffolds; tissue mechanics; nanotubes; tissue stretcher; tissue elasticity; porcine eyes
Show Figures

Graphical abstract

MDPI and ACS Style

Juncheed, K.; Kohlstrunk, B.; Friebe, S.; Dallacasagrande, V.; Maurer, P.; Reichenbach, A.; Mayr, S.G.; Zink, M. Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. Int. J. Mol. Sci. 2020, 21, 3889. https://doi.org/10.3390/ijms21113889

AMA Style

Juncheed K, Kohlstrunk B, Friebe S, Dallacasagrande V, Maurer P, Reichenbach A, Mayr SG, Zink M. Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension. International Journal of Molecular Sciences. 2020; 21(11):3889. https://doi.org/10.3390/ijms21113889

Chicago/Turabian Style

Juncheed, Kantida, Bernd Kohlstrunk, Sabrina Friebe, Valentina Dallacasagrande, Patric Maurer, Andreas Reichenbach, Stefan G. Mayr, and Mareike Zink. 2020. "Employing Nanostructured Scaffolds to Investigate the Mechanical Properties of Adult Mammalian Retinae Under Tension" International Journal of Molecular Sciences 21, no. 11: 3889. https://doi.org/10.3390/ijms21113889

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop