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Open AccessArticle

Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts

1
Department of Neuroscience, Imaging and clinical Sciences—Center for Advanced Studies and Technology (CAST), University G. d’Annunzio of Chieti-Pescara, 06100 Chieti, Italy
2
Department of Surgery “Pietro Valdoni”, Sapienza University of Rome, 00161 Rome, Italy
3
Azienda Policlinico Umberto I, 00161 Rome, Italy
4
Department of Experimental Medicine, Sapienza University of Rome, Systems Biology Group Lab, 00161 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(10), 3638; https://doi.org/10.3390/ijms21103638
Received: 13 April 2020 / Revised: 18 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Microgravity and Space Medicine)
The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteoblastic cells. Using the model of murine MC3T3-E1 osteoblast cells, cellular morphology, proliferation, and metabolism were investigated during exposure to simulated microgravity on a random positioning machine in the absence or presence of an antioxidant—the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our results confirm that simulated microgravity-induced morphological and metabolic alterations characterized by increased levels of reactive oxygen species and a slowdown of the proliferative rate. Interestingly, the use of Trolox inhibited the simulated microgravity-induced effects. Indeed, the antioxidant-neutralizing oxidants preserved cell cytoskeletal architecture and restored cell proliferation rate and metabolism. The use of appropriate antioxidant countermeasures could prevent the modifications and damage induced by microgravity on osteoblastic cells and consequently on bone homeostasis. View Full-Text
Keywords: osteoblasts; microgravity; random positioning machine; oxidative stress; intracellular calcium osteoblasts; microgravity; random positioning machine; oxidative stress; intracellular calcium
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MDPI and ACS Style

Morabito, C.; Guarnieri, S.; Cucina, A.; Bizzarri, M.; Mariggiò, M.A. Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts. Int. J. Mol. Sci. 2020, 21, 3638.

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