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Open AccessArticle

The Effect of Proline cis-trans Isomerization on the Folding of the C-Terminal SH2 Domain from p85

1
Istituto Pasteur—Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche “A. Rossi Fanelli”, 00161 Roma, Italy
2
Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, 00185 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 125; https://doi.org/10.3390/ijms21010125
Received: 15 October 2019 / Revised: 20 December 2019 / Accepted: 20 December 2019 / Published: 23 December 2019
(This article belongs to the Special Issue Protein Folding and Misfolding ---- Structure and Functions)
SH2 domains are protein domains that modulate protein–protein interactions through a specific interaction with sequences containing phosphorylated tyrosines. In this work, we analyze the folding pathway of the C-terminal SH2 domain of the p85 regulatory subunit of the protein PI3K, which presents a proline residue in a cis configuration in the loop between the βE and βF strands. By employing single and double jump folding and unfolding experiments, we demonstrate the presence of an on-pathway intermediate that transiently accumulates during (un)folding. By comparing the kinetics of folding of the wild-type protein to that of a site-directed variant of C-SH2 in which the proline was replaced with an alanine, we demonstrate that this intermediate is dictated by the peptidyl prolyl cis-trans isomerization. The results are discussed in the light of previous work on the effect of peptidyl prolyl cis-trans isomerization on folding events. View Full-Text
Keywords: protein folding; misfolding; kinetics; mutagenesis protein folding; misfolding; kinetics; mutagenesis
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MDPI and ACS Style

Troilo, F.; Malagrinò, F.; Visconti, L.; Toto, A.; Gianni, S. The Effect of Proline cis-trans Isomerization on the Folding of the C-Terminal SH2 Domain from p85. Int. J. Mol. Sci. 2020, 21, 125. https://doi.org/10.3390/ijms21010125

AMA Style

Troilo F, Malagrinò F, Visconti L, Toto A, Gianni S. The Effect of Proline cis-trans Isomerization on the Folding of the C-Terminal SH2 Domain from p85. International Journal of Molecular Sciences. 2020; 21(1):125. https://doi.org/10.3390/ijms21010125

Chicago/Turabian Style

Troilo, Francesca; Malagrinò, Francesca; Visconti, Lorenzo; Toto, Angelo; Gianni, Stefano. 2020. "The Effect of Proline cis-trans Isomerization on the Folding of the C-Terminal SH2 Domain from p85" Int. J. Mol. Sci. 21, no. 1: 125. https://doi.org/10.3390/ijms21010125

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