Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa
Chemical Engineering Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430 Guadalajara, Jalisco, Mexico
Electronics Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán #1421, C.P. 44430 Guadalajara, Jalisco, Mexico
Wood, Pulp and Paper Department, Universidad de Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales, Las Aguilas, C.P. 45010 Zapopan, Jalisco, Mexico
Author to whom correspondence should be addressed.
Received: 27 February 2019 / Revised: 13 April 2019 / Accepted: 16 April 2019 / Published: 23 April 2019
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Anthropogenic activities have increased the amount of urban wastewater discharged into natural aquatic reservoirs containing a high amount of nutrients such as phosphorus (Pi and PO
), nitrogen (NH
) and organic contaminants. Most of the urban wastewater in Mexico do not receive any treatment to remove nutrients. Several studies have reported that an alternative to reduce those contaminants is using consortiums of microalgae and endogenous bacteria. In this research, a genome-scale biochemical reaction network is reconstructed for the co-culture between the microalga Chlorella vulgaris
and the bacterium Pseudomonas aeruginosa
. Metabolic Pathway Analysis (MPA), is applied to understand the metabolic capabilities of the co-culture and to elucidate the best conditions in removing nutrients. Theoretical yields for phosphorus removal under photoheterotrophic conditions are calculated, determining their values as 0.042 mmol of PO
per g DW of C. vulgaris
, 19.43 mmol of phosphorus (Pi) per g DW of C. vulgaris
and 4.90 mmol of phosphorus (Pi) per g DW of P. aeruginosa
. Similarly, according to the genome-scale biochemical reaction network the theoretical yields for nitrogen removal are 10.3 mmol of NH
per g DW of P. aeruginosa
and 7.19 mmol of NO
per g DW of C. vulgaris
. Thus, this research proves the metabolic capacity of these microorganisms in removing nutrients and their theoretical yields are calculated.
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Guerra-Renteria, A.S.; García-Ramírez, M.A.; Gómez-Hermosillo, C.; Gómez-Guzmán, A.; González-García, Y.; González-Reynoso, O. Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa. Int. J. Mol. Sci. 2019, 20, 1978.
Guerra-Renteria AS, García-Ramírez MA, Gómez-Hermosillo C, Gómez-Guzmán A, González-García Y, González-Reynoso O. Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa. International Journal of Molecular Sciences. 2019; 20(8):1978.
Guerra-Renteria, A. S.; García-Ramírez, M. A.; Gómez-Hermosillo, César; Gómez-Guzmán, Abril; González-García, Yolanda; González-Reynoso, Orfil. 2019. "Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa." Int. J. Mol. Sci. 20, no. 8: 1978.
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