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Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa

1
Chemical Engineering Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán # 1451, C.P. 44430 Guadalajara, Jalisco, Mexico
2
Electronics Department, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. M. García Barragán #1421, C.P. 44430 Guadalajara, Jalisco, Mexico
3
Wood, Pulp and Paper Department, Universidad de Guadalajara, Km. 15.5 Carretera Guadalajara-Nogales, Las Aguilas, C.P. 45010 Zapopan, Jalisco, Mexico
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(8), 1978; https://doi.org/10.3390/ijms20081978
Received: 27 February 2019 / Revised: 13 April 2019 / Accepted: 16 April 2019 / Published: 23 April 2019
(This article belongs to the Special Issue Extreme Microorganisms in the Nitrogen Cycle)
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Abstract

Anthropogenic activities have increased the amount of urban wastewater discharged into natural aquatic reservoirs containing a high amount of nutrients such as phosphorus (Pi and PO 4 3 ), nitrogen (NH 3 and NO 3 ) and organic contaminants. Most of the urban wastewater in Mexico do not receive any treatment to remove nutrients. Several studies have reported that an alternative to reduce those contaminants is using consortiums of microalgae and endogenous bacteria. In this research, a genome-scale biochemical reaction network is reconstructed for the co-culture between the microalga Chlorella vulgaris and the bacterium Pseudomonas aeruginosa. Metabolic Pathway Analysis (MPA), is applied to understand the metabolic capabilities of the co-culture and to elucidate the best conditions in removing nutrients. Theoretical yields for phosphorus removal under photoheterotrophic conditions are calculated, determining their values as 0.042 mmol of PO 4 3 per g DW of C. vulgaris, 19.43 mmol of phosphorus (Pi) per g DW of C. vulgaris and 4.90 mmol of phosphorus (Pi) per g DW of P. aeruginosa. Similarly, according to the genome-scale biochemical reaction network the theoretical yields for nitrogen removal are 10.3 mmol of NH 3 per g DW of P. aeruginosa and 7.19 mmol of NO 3 per g DW of C. vulgaris. Thus, this research proves the metabolic capacity of these microorganisms in removing nutrients and their theoretical yields are calculated. View Full-Text
Keywords: extreme pathways; nutrient removal; C. vulgaris; P. aeruginosa extreme pathways; nutrient removal; C. vulgaris; P. aeruginosa
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Guerra-Renteria, A.S.; García-Ramírez, M.A.; Gómez-Hermosillo, C.; Gómez-Guzmán, A.; González-García, Y.; González-Reynoso, O. Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. vulgaris and P. aeruginosa. Int. J. Mol. Sci. 2019, 20, 1978.

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