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Open AccessArticle

Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference

1
Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA
2
James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
3
Molecular Targets Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
4
Department of Medicine, University of Louisville, Louisville, KY 40202, USA
5
Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1235; https://doi.org/10.3390/ijms20051235
Received: 19 February 2019 / Revised: 7 March 2019 / Accepted: 8 March 2019 / Published: 12 March 2019
(This article belongs to the Special Issue Focus on Exosome-Based Cell-Cell Communication in Health and Disease)
Melanoma-derived small extracellular vesicles (sEVs) participate in tumor pathogenesis. Tumor pathogenesis is highly dependent on inflammatory processes. Given the potential for melanoma sEVs to carry tumor biomarkers, we explored the hypothesis that they may contain inflammation-related mRNA content. Biophysical characterization showed that human primary melanocyte-derived sEVs trended toward being smaller and having less negative (more neutral) zeta potential than human melanoma sEVs (A-375, SKMEL-28, and C-32). Using primary melanocyte sEVs as the control population, RT-qPCR array results demonstrated similarities and differences in gene expression between melanoma sEV types. Upregulation of pro-angiogenic chemokine ligand CXCL1, CXCL2, and CXCL8 mRNAs in A-375 and SKMEL-28 melanoma sEVs was the most consistent finding. This paralleled increased production of CXCL1, CXCL2, and CXCL8 proteins by A-375 and SKMEL-28 sEV source cells. Overall, the use of primary melanocyte sEVs as a control sEV reference population facilitated the detection of inflammation-related melanoma sEV mRNA content. View Full-Text
Keywords: melanoma; extracellular vesicle; exosome; inflammation; mRNA; biomarker melanoma; extracellular vesicle; exosome; inflammation; mRNA; biomarker
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Bardi, G.T.; Al-Rayan, N.; Richie, J.L.; Yaddanapudi, K.; Hood, J.L. Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference. Int. J. Mol. Sci. 2019, 20, 1235.

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