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Open AccessArticle

Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana

1
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G2W1, Canada
2
Noble Research Institute, Limited Liability Company (LLC), 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
3
Department of Plant Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115 Bonn, Germany
4
School of Biological Sciences, University of Western Australia, 35 Stirling Highway, Crawly, Perth, WA 6009, Australia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6117; https://doi.org/10.3390/ijms20246117
Received: 24 October 2019 / Revised: 27 November 2019 / Accepted: 2 December 2019 / Published: 4 December 2019
(This article belongs to the Section Molecular Plant Sciences)
NAC (NAM (no apical meristem), ATAF1/2, and CUC2 (cup-shaped cotyledon)) proteins are one of the largest families of plant-specific transcription factors, and this family is present in a wide range of land plants. Here, we have investigated the role of ANAC046 in the regulation of suberin biosynthesis and deposition in Arabidopsis. Subcellular localization and transcriptional activity assays showed that ANAC046 localizes in the nucleus, where it functions as a transcription activator. Analysis of the PANAC046:GUS lines revealed that ANAC046 is mainly expressed in the root endodermis and periderm, and is also induced in leaves by wounding. The transgenic lines overexpressing ANAC046 exhibited defective surfaces on the aerial plant parts compared to the wild-type (WT) as characterized by increased permeability for Toluidine blue stain and greater chlorophyll leaching. Quantitative RT-PCR analysis showed that the expression of suberin biosynthesis genes was significantly higher in the roots and leaves of overexpression lines compared to the WT. The biochemical analysis of leaf cuticular waxes showed that the overexpression lines accumulated 30% more waxes than the WT. Concurrently, overexpression lines also deposited almost twice the amount of suberin content in their roots compared with the WT. Taken together, these results showed that ANAC046 is an important transcription factor that promotes suberin biosynthesis in Arabidopsis thaliana roots. View Full-Text
Keywords: ANAC046; Arabidopsis thaliana; permeability; suberin biosynthesis; transcription activator; roots ANAC046; Arabidopsis thaliana; permeability; suberin biosynthesis; transcription activator; roots
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Mahmood, K.; Zeisler-Diehl, V.V.; Schreiber, L.; Bi, Y.-M.; Rothstein, S.J.; Ranathunge, K. Overexpression of ANAC046 Promotes Suberin Biosynthesis in Roots of Arabidopsis thaliana. Int. J. Mol. Sci. 2019, 20, 6117.

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