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Open AccessArticle

Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains

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Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg, Hollystr. 1, D-06114 Halle/S., Germany
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Institut für Chemie—Lebensmittelchemie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Str.2, D-06120 Halle/S., Germany
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Leibniz Institute on Aging—Fritz Lipmann Institut, Beutenbergstr. 11, 07745 Jena, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6118; https://doi.org/10.3390/ijms20246118
Received: 31 October 2019 / Revised: 26 November 2019 / Accepted: 2 December 2019 / Published: 4 December 2019
Aging represents the accumulation of changes in an individual over time, encompassing physical, psychological, and social changes. Posttranslational modifications of proteins such as glycosylation, including sialylation or glycation, are proposed to be involved in this process, since they modulate a variety of molecular and cellular functions. In this study, we analyzed selected posttranslational modifications and the respective proteins on which they occur in young and old mouse brains. The expression of neural cell adhesion molecule (NCAM), receptor for advanced glycation endproducts (RAGE), as well as the carbohydrate-epitopes paucimannose and high-mannose, polysialic acid, and O-GlcNAc were examined. We demonstrated that mannose-containing glycans increased on glycoproteins in aged mouse brains and identified synapsin-1 as one major carrier of paucimannose in aged brains. In addition, we found an accumulation of so-called advanced glycation endproducts, which are generated by non-enzymatic reactions and interfere with protein function. Furthermore, we analyzed the expression of sialic acid and found also an increase during aging. View Full-Text
Keywords: sialic acid; neural cell adhesion molecule (NCAM), glycation; O-GlcNAc; mannose sialic acid; neural cell adhesion molecule (NCAM), glycation; O-GlcNAc; mannose
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Simon, F.; Bork, K.; Gnanapragassam, V.S.; Baldensperger, T.; Glomb, M.A.; Di Sanzo, S.; Ori, A.; Horstkorte, R. Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains. Int. J. Mol. Sci. 2019, 20, 6118.

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