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TASK-3 Gene Knockdown Dampens Invasion and Migration and Promotes Apoptosis in KATO III and MKN-45 Human Gastric Adenocarcinoma Cell Lines

1
Center for Medical Research, School of Medicine, Universidad de Talca, Talca 3460000, Chile
2
Programa de Investigación Asociativa en Cáncer Gástrico (PIA-CG), Universidad de Talca, Talca 3460000, Chile
3
Lymphatic and Inflammation Research Laboratory, Faculty of Health Sciences, Institute of Biomedical Science, Universidad Autónoma de Chile, Talca 3467987, Chile
4
Center for Bioinformatics and Molecular Simulations (CBSM), Universidad de Talca, Talca 3460000, Chile
5
Millennium Nucleus of Ion Channels-Associated Diseases (MiNICAD), Universidad de Talca, Talca 3460000, Chile
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6077; https://doi.org/10.3390/ijms20236077
Received: 22 October 2019 / Revised: 26 November 2019 / Accepted: 27 November 2019 / Published: 2 December 2019
(This article belongs to the Section Molecular Oncology)
Incidence and mortality of gastric cancer is increasing worldwide, in part, because of the lack of new therapeutic targets to treat this disease. Different types of ion channels participate in the hallmarks of cancer. In this context, ion channels are known to exert control over the cell cycle, mechanisms that support survival, angiogenesis, migration, and cell invasion. In particular, TASK-3 (KCNK9), a member of the K2P potassium channel family, has attracted much interest because of its oncogenic properties. However, despite multiple lines of evidence linking TASK-3 to tumorigenesis in various types of cancer, its relationship with gastric cancer has not been fully examined. Therefore, we set out to assess the effect of TASK-3 gene knockdown on KATO III and MKN-45 human gastric adenocarcinoma cell lines by using a short hairpin RNA (shRNA)-mediated knockdown. Our results demonstrate that knocking down TASK-3 reduces cell proliferation and viability because of an increase in apoptosis without an apparent effect on cell cycle checkpoints. In addition, cell migration and invasion are reduced after knocking down TASK-3 in these cell lines. The present study highlights TASK-3 as a key protein involved in migration and cell survival in gastric cancer and corroborates its potential as a therapeutic target for gastric cancer treatment. View Full-Text
Keywords: TASK-3; gastric cancer; cell proliferation; apoptosis; migration; invasion TASK-3; gastric cancer; cell proliferation; apoptosis; migration; invasion
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Cikutović-Molina, R.; Herrada, A.A.; González, W.; Brown, N.; Zúñiga, L. TASK-3 Gene Knockdown Dampens Invasion and Migration and Promotes Apoptosis in KATO III and MKN-45 Human Gastric Adenocarcinoma Cell Lines. Int. J. Mol. Sci. 2019, 20, 6077.

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