Depletion of KNL2 Results in Altered Expression of Genes Involved in Regulation of the Cell Cycle, Transcription, and Development in Arabidopsis
by
Anastassia Boudichevskaia 1,*
, Andreas Houben 1, Anne Fiebig 1, Klara Prochazkova 2, Ales Pecinka 2 and Inna Lermontova 1,3,*
Anastassia Boudichevskaia 1,*, Andreas Houben 1, Anne Fiebig 1, Klara Prochazkova 2, Ales Pecinka 2 and Inna Lermontova 1,3,*
1
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstrasse 3, D-06466 Seeland, Germany
2
Institute of Experimental Botany, Czech Acad Sci, Centre of the Region Haná for Biotechnological and Agricultural Research (CRH), Šlechtitelů 31, CZ-77900 Olomouc, Czech Republic
3
Mendel Centre for Plant Genomics and Proteomics, CEITEC, Masaryk University, Brno CZ-62500, Czech Republic
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(22), 5726; https://doi.org/10.3390/ijms20225726
Received: 10 September 2019 / Revised: 7 November 2019 / Accepted: 11 November 2019 / Published: 15 November 2019
(This article belongs to the Special Issue Chromatin, Epigenetics and Plant Physiology)
Centromeres contain specialized nucleosomes at which histone H3 is partially replaced by the centromeric histone H3 variant cenH3 that is required for the assembly, maintenance, and proper function of kinetochores during mitotic and meiotic divisions. Previously, we identified a KINETOCHORE NULL 2 (KNL2) of Arabidopsis thaliana that is involved in the licensing of centromeres for the cenH3 recruitment. We also demonstrated that a knockout mutant for KNL2 shows mitotic and meiotic defects, slower development, reduced growth rate, and fertility. To analyze an effect of KNL2 mutation on global gene transcription of Arabidopsis, we performed RNA-sequencing experiments using seedling and flower bud tissues of knl2 and wild-type plants. The transcriptome data analysis revealed a high number of differentially expressed genes (DEGs) in knl2 plants. The set was enriched in genes involved in the regulation of the cell cycle, transcription, development, and DNA damage repair. In addition to comprehensive information regarding the effects of KNL2 mutation on the global gene expression, physiological changes in plants are also presented, which provides an integrated understanding of the critical role played by KNL2 in plant growth and development.
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Keywords:
Arabidopsis; KNL2; kinetochores; RNA-seq; centromere
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Boudichevskaia, A.; Houben, A.; Fiebig, A.; Prochazkova, K.; Pecinka, A.; Lermontova, I. Depletion of KNL2 Results in Altered Expression of Genes Involved in Regulation of the Cell Cycle, Transcription, and Development in Arabidopsis. Int. J. Mol. Sci. 2019, 20, 5726.
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