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Open AccessArticle

Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury

Division of Experimental and Clinical Cardiology, Research Institute for Complex Issues of Cardiovascular Diseases, 6 Sosnovy Boulevard, Kemerovo 650002, Russia
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Int. J. Mol. Sci. 2019, 20(22), 5728; https://doi.org/10.3390/ijms20225728
Received: 10 October 2019 / Revised: 11 November 2019 / Accepted: 13 November 2019 / Published: 15 November 2019
(This article belongs to the Special Issue Pathomechanisms of Atherosclerosis. Part II)
Calcium phosphate bions (CPBs) are formed under blood supersaturation with calcium and phosphate owing to the mineral chaperone fetuin-A and representing mineralo-organic particles consisting of bioapatite and multiple serum proteins. While protecting the arteries from a rapid medial calcification, CPBs cause endothelial injury and aggravate intimal hyperplasia in balloon-injured rat aortas. Here, we asked whether CPBs induce intimal hyperplasia in intact rat arteries in the absence of cardiovascular risk factors. Normolipidemic Wistar rats were subjected to regular (once/thrice per week over 5 weeks) tail vein injections of either spherical (CPB-S) or needle-shaped CPBs (CPB-N), magnesium phosphate bions (MPBs), or physiological saline (n = 5 per group). Neointima was revealed in 3/10 and 4/10 rats which received CPB-S or CPB-N, respectively, regardless of the injection regimen or blood flow pattern in the aortic segments. In contrast, none of the rats treated with MPBs or physiological saline had intimal hyperplasia. The animals also did not display signs of liver or spleen injury as well as extraskeletal calcium deposits. Serum alanine/aspartate transaminases, interleukin-1β, MCP-1/CCL2, C-reactive protein, and ceruloplasmin levels did not differ among the groups. Hence, CPBs may provoke intimal hyperplasia via direct endothelial injury regardless of their shape or type of blood flow. View Full-Text
Keywords: bions; calciprotein particles; calcium phosphate; hydroxyapatite; cardiovascular disease; blood flow; intimal hyperplasia; neointima; endothelial dysfunction; endothelial injury bions; calciprotein particles; calcium phosphate; hydroxyapatite; cardiovascular disease; blood flow; intimal hyperplasia; neointima; endothelial dysfunction; endothelial injury
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MDPI and ACS Style

Shishkova, D.; Velikanova, E.; Sinitsky, M.; Tsepokina, A.; Gruzdeva, O.; Bogdanov, L.; Kutikhin, A. Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury. Int. J. Mol. Sci. 2019, 20, 5728. https://doi.org/10.3390/ijms20225728

AMA Style

Shishkova D, Velikanova E, Sinitsky M, Tsepokina A, Gruzdeva O, Bogdanov L, Kutikhin A. Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury. International Journal of Molecular Sciences. 2019; 20(22):5728. https://doi.org/10.3390/ijms20225728

Chicago/Turabian Style

Shishkova, Daria; Velikanova, Elena; Sinitsky, Maxim; Tsepokina, Anna; Gruzdeva, Olga; Bogdanov, Leo; Kutikhin, Anton. 2019. "Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury" Int. J. Mol. Sci. 20, no. 22: 5728. https://doi.org/10.3390/ijms20225728

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